HPLC Analysis of Warfarin Enantiomers on Astec^® CHIROBIOTIC^® V (Method 1)

column

CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)

mobile phase

[A] acetonitrile; [B] 5 mM ammonium acetate, pH 4.1; (30:70, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

5 μL

sample

1 mg/mL in mobile phase