Analiza HPLC taksoli na urządzeniu 2445 Express F5

column

Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)

mobile phase

[A] water; [B] acetonitrile; (60:40, A:B)

flow rate

0.3 mL/min

column temp.

30 °C

detector

UV, 227 nm

injection

2 μL

sample

25 mg/L in 70:30, water:methanol