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Merck

HPLC Analysis of Levothyroxine and Liothyronine on Ascentis® Express ES-Cyano

HPLC Analysis of Levothyroxine and Liothyronine on Ascentis® Express ES-Cyano application for HPLC

Materiały

kolumna analityczna

Numer produktu
Opis
Cennik

53491-U

Ascentis® Express ES-Cyano, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 4.6 mm

standardowe

Numer produktu
Opis
Cennik

T6397

3,3′,5-Triiodo-L-thyronine sodium salt

powder, BioReagent, suitable for cell culture

32995

Levothyroxine sodium hydrate

PESTANAL®, analytical standard

CONDITIONS

column

Ascentis Express ES-Cyano, 10 cm x 2.1 mm I.D., 2.7 μm particles (53491-U)

mobile phase

[A] water with 0.05% phosphoric acid; [B] acetonitrile with 0.05% phophoric acid; (60:40, A:B)

flow rate

1.5 mL/min

pressure

3270 psi (225 bar)

column temp.

30 °C

detector

UV, 225 nm

injection

5 μL

sample

20 μg/mL in mobile phase

Opis

Komentarz do analizy

This application demonstrates the suitabilty of Ascentis Express ES-Cyano for the analysis of Levothyroxine according to USP Monograph 3301 with Liothyronine as a reference standard.

Informacje prawne

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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