HPLC Analysis of AQC-Alanine Enantiomers on Astec® CHIROBIOTIC® T2
CONDITIONS
column
CHIROBIOTIC T2, 25 cm x 4.6 mm I.D., 5 μm particles (16024AST)
mobile phase
[A] 20 mM ammonium acetate, pH 4.1; [B] methanol; (50:50, A:B)
flow rate
0.9 mL/min
column temp.
25 °C
detector
UV, 254 nm
injection
0.5 μL
sample
2 mg/mL in methanol
Opis
Informacje prawne
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC