GC Analysis of Basic Drugs on SLB^®-5ms (20 m x 0.18 mm I.D., 0.36 μm) after SPME using 65 μm PDMS/DVB Fiber, Fast GC Analysis

sample/matrix

500 μg/L of each compound in 2 mL 0.05M phosphate buffer, 25% sodium chloride, pH=11

SPME fiber

Polyacrylate, 85 μm (57304); PDMS/DVB Stableflex, 65 μm (57326-U)

extraction

15 min, fiber immersion in water, room temperture, stirring at 450 rpm

desorption process

2 min, 250 °C

column

SLB-5ms, 20 m x 0.18 mm I.D., 0.36 μm (28576-U)

oven

100 °C (1 min), 20 °C/min to 325 °C (5 min)

detector

MSD, m/z 40 - 450

MSD interface

325 °C

carrier gas

helium, 1.5 mL/min, constant

liner

0.75 mm I.D. SPME