G003760

GC Analysis of FAMEs in Popcorn Oil on SP^®-2560 (75 m x 0.18 mm I.D., 0.14 μm) after SPE Fractionation using Discovery^® Ag-Ion, Fast GC Analysis

application for SPE, suitable for GC

Właściwości

• metody: gas chromatography (GC): suitable; solid phase extraction (SPE): suitable
• parametry testowe: sample preparation: SPE (Solid Phase Extraction); sample/matrix: 1 g of popcorn oil FAME′s in Hexane; SPE tube/cartridge: Discovery Ag-Ion, 750 mg/6 mL (54225-U); condition: 4 mL acetone and 4 mL hexane; sample addition: 1 mL of the hexane FAME extract; elution: 6 mL hexane:acetone (96:4, v/v) followed by 4 mL hexane:acetone (90:10, v/v); column: SP-2560, 75 m x 0.18mm I.D., 0.14 μm (23348-U); oven: 180 °C isothermal; inj. temp.: 220 °C; detector: FID, 220 °C; carrier gas: hydrogen, 40 cm/sec. at 180 °C; injection: (0.5 μL, 100:1 split); liner: 0.5 μL, 100:1 split
• przydatność: application for SPE
• Zastosowanie: agriculture; food and beverages

Opis

Uwaga dotycząca przygotowania

Sample pretreatment: A 1 g sample of popcorn oil was scraped from the inside of a popped microwave oven popcorn bag and mixed with 8 mL deionized water (DI) and 4 mL petroleum ether, centrifuged to remove particulates. The ether layer (upper layer) was collected. This petroleum ether extraction step was repeated four times. The upper ether layers were combined, evaporated, and reconstituted in 16 mL toluene. A 1 mL aliquot was transferred to conical reaction vial and 2 mL 7% boron triflluoride in methanol were added. The reaction was incubated at 80 °C for 15 minutes using a heating block, then cooled to room temperature. 1 mL DI water was added and FAMEs were extracted twice using 1 mL hexane each time. The upper hexane layers were combined into a fresh vial, evaporated, and reconstituted in 5 mL hexane and 50 mg anhydrous sodium sulfate.

Komentarz do analizy

Discovery Ag-Ion działa w oparciu o chromatografię argentacyjną, która jest potężną techniką analityczną i przygotowywania próbek do separacji lub frakcjonowania cis i trans geometrycznych i pozycyjnych kwasów tłuszczowych (FA), estrów metylowych kwasów tłuszczowych (FAME), diacylogliceroli (DAG) i izomerów triacylogliceroli (TAG). Discovery Ag-Ion SPE pokazano w tym zastosowaniu do oddzielania FAME z próbki oleju jadalnego.

Informacje prawne

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

SP is a registered trademark of Sigma-Aldrich Co. LLC

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