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ZRB2984

Sigma-Aldrich

Anti-Perforin-1 Antibody, clone 1H11 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonim(y):

Cytolysin, Lymphocyte pore-forming protein, PFP

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.43

pochodzenie biologiczne

rabbit

Poziom jakości

rekombinowane

expressed in HEK 293 cells

białko sprzężone

unconjugated

forma przeciwciała

purified antibody

rodzaj przeciwciała

primary antibodies

klon

1H11, recombinant monoclonal

opis

recombinant, expressed in HEK 293 cells

linia produktu

ZooMAb® learn more

Postać

lyophilized

masa cząsteczkowa

calculated mol wt 61.38 kDa
observed mol wt ~57 kDa

oczyszczone przez

using Protein A

reaktywność gatunkowa

human

opakowanie

antibody small pack of 25

charakterystyka ekologicznej alternatywy

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

rozszerzona walidacja

recombinant expression
Learn more about Antibody Enhanced Validation

metody

affinity binding assay: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

izotyp

IgG

sekwencja epitopowa

Internal

numer dostępu Protein ID

numer dostępu UniProt

kategoria ekologicznej alternatywy

Warunki transportu

ambient

temp. przechowywania

2-8°C

informacje o genach

human ... PRF1(5551)

Opis ogólny

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specyficzność

Clone 1H11 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Perforin-1. It targets an epitope within 19 amino acids from the internal region.

Immunogen

KLH-conjugated linear peptide corresponding to 19 amino acids from the internal region of human Perforin-1.

Zastosowanie

Quality Control Testing

Evaluated by Western Blotting in NK-92 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Perforin-1 in NK-92 cell lysate.

Tested Applications

Flow Cytometry Analysis: 1 µg from a representative lot detected Perforin-1 in one million Human peripheral blood mononuclear cells (PBMC)..

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Perforin-1 in Human spleen tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Perforin-1 peptide with a KD of 5.8 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Opis wartości docelowych

Perforin-1 (UniProt: P14222; also known as P1, Cytolysin, Lymphocyte pore-forming protein, PFP) is encoded by the PRF1 (also known as PFP) gene (Gene ID: 5551) in human. Perforin-1 is a multi-pass membrane glycoprotein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature form that contains two transmembrane domains (aa 129-149 and 257-279). It is stored in cytolytic granules of cytolytic T-lymphocytes and is secreted into the cleft between T-lymphocyte and target cell. Perforin has a thin key-shaped structure consisting of an N-terminal membrane attack complex perforin-like (MACPF)/cholesterol dependent cytolysin (CDC) domain (aa 27-375), which is followed by an EGF domain (aa 376-408) that together with the extreme C-terminal sequence forms a central shelf-like structure. Its C-terminal C2 domain (aa 397-519) mediates initial, Ca2+-dependent membrane binding. Perforin-1 serves as a pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. It also plays an important role in killing other cells that are recognized as non-self by the immune system. It can insert into the membrane of target cells in its calcium-bound form and can oligomerize and form large pores. Its membrane insertion and pore formation require a major conformation change. It promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. Mutations in PRF1 gene have been linked to familial Hemophagocytic lymphohistiocytosis (FHL2), a rare disorder that is characterized by immune dysregulation with hypercytokinemia, defective function of natural killer cell, and massive infiltration of several organs by activated lymphocytes and macrophages. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Praper, T., et al. (2011). J. Biol. Chem. 286(4); 2946-2955; Law, RHP., et al. (2010). Nature. 468(7322). 447-451; Ando, K., et al. (1997). J. Immunol. 158(11); 5283-5291).

Postać fizyczna

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Rekonstytucja

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informacje prawne

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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