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ZRB1493

Sigma-Aldrich

Anti-Nrf2 Antibody, clone 5B20 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonim(y):

HEBP1, NF-E2-related factor 2, NFE2-related factor 2, Nuclear factor, Nuclear factor erythroid 2-related factor 2, erythroid derived 2 like 2

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
12352203
NACRES:
NA.43

pochodzenie biologiczne

rabbit (recombinant)

Poziom jakości

rekombinowane

expressed in HEK 293 cells

białko sprzężone

unconjugated

forma przeciwciała

purified antibody

rodzaj przeciwciała

primary antibodies

klon

5B20, monoclonal
recombinant monoclonal

linia produktu

ZooMAb® learn more

Postać

lyophilized

masa cząsteczkowa

calculated mol wt 67.83 kDa
observed mol wt 80 kDa

reaktywność gatunkowa

human

opakowanie

antibody small pack of 25 μL

charakterystyka ekologicznej alternatywy

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

rozszerzona walidacja

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

metody

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

izotyp

IgG

numer dostępu UniProt

kategoria ekologicznej alternatywy

Warunki transportu

ambient

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... NFE2L2(4780)

Opis ogólny

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specyficzność

Clone 5B20 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Nrf2. It targets an epitope within 15 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Nuclear factor erythroid 2-related factor 2 (Nrf2).

Zastosowanie

Anti-Nrf2, clone 5B20 ZooMAb, Cat. No. ZRB1493, is a recombinant Rabbit monoclonal antibody that specifically targets Nrf2 (NFE2-related factor 2) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Nrf2 in human gallbladder and human cervical cancer tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Nrf2 in MG132 treated HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Opis wartości docelowych

Nuclear factor erythroid 2-related factor 2 (UniProt: Q16236; also known as NF-E2-related factor 2, NFE2-related factor 2, Nrf-2, HEBP1, Nuclear factor, erythroid derived 2, like 2) is encoded by the NFE2L2 (also known as NRF2) gene (Gene ID: 4780) in human. Nrf2 is a cap n collar basic-region leucine zipper transcription factor that that heterodimerizes with small Maf proteins. It is involved in adaptation of cells to oxidative stress and electrophiles. It binds to antioxidant response (ARE) elements in the promoter regions of target genes and induces a set of cytoprotective genes that are involved in the synthesis of reduced glutathione (GSH), repair of oxidized protein thiols, scavenging of reactive oxygen species, and drug metabolism. It is cytosolic under unstressed conditions, but translocates into the nucleus upon induction by electrophilic agents. Under normal unstressed conditions it is rapidly turned over in a Keap-1 dependent manner through Cul3-Rbx1 ubiquitination and proteasomal degradation. Phosphorylation of Ser40 by protein kinase C in response to oxidative stress dissociates Nrf2 from its Keap-1 and promotes its translocation into the nucleus. It is widely expressed with highest expression in liver, skeletal muscle, luminal cells of the stomach and intestine, lining of the bronchi and alveoli, and in renal tubules; followed by heart, spleen, testis and brain. Nrf2 is constitutively upregulated in many tumors due to somatic mutations in the Keap1 or Nrf2 genes. Tumors over-expressing Nrf2 exhibit increased resistance to chemotherapeutic drugs and higher rates of proliferation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hayes, JD., and Ashford, MLJ (2012). Cell Metab. 16(2): 139-141).

Postać fizyczna

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Rekonstytucja

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informacje prawne

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

13 - Non Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 2


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