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SRP2003

Sigma-Aldrich

TBP (TATA box binding protein) human

recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

Synonim(y):

GTF2D, GTF2D1, HDL4, MGC117320, TFIID

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About This Item

Kod UNSPSC:
12352202
NACRES:
NA.77

pochodzenie biologiczne

human

rekombinowane

expressed in E. coli

Próba

≥85% (SDS-PAGE)

Formularz

frozen liquid

masa cząsteczkowa

~39 kDa

opakowanie

pkg of 10 μg

warunki przechowywania

avoid repeated freeze/thaw cycles

stężenie

200 μg/mL

metody

electrophoretic mobility shift assay: suitable

kolor

clear colorless

numer dostępu NCBI

NM_003194

numer dostępu UniProt

P20226

Warunki transportu

dry ice

temp. przechowywania

−70°C

informacje o genach

human ... TBP(6908)

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Opis ogólny

TBP (TATA-box binding protein) was originally recognized as a part of the class II initiation factor TFIID. Its C-terminal region is phylogenetically conserved, and is composed of 180-amino acids, containing a highly basic segment, the basic repeat, flanked by two direct repeats. The N-terminal domain of this protein is not conserved across species, and differs in number of residues. TBP gene is localized to human chromosome 6.

Działania biochem./fizjol.

TBP (TATA-box binding protein) is essential for the optimal initiation of transcription of ribosomal, messenger, small nuclear, and transfer RNAs by all three eukaryotic RNA polymerases. TBP proteins binds to the TATA consensus sequence (TATAa/tAa/t) with high affinity, through its C-terminal or core region, and identifies minor groove segments and introduces significant DNA deformation. It is a component of the class II initiation factor TFIID, along with TBP-associated factors (TAFIIs), which is crucial for nucleating the assembly of Pol II pre-initiation complex (PIC). PIC is essential for the transcription initiation by RNA polymerase II (Pol II). Amplification of the CAG/CAA trinucleotide repeats in TBP gene results in an autosomal dominant cerebellar ataxia, SCA17 (spinocerebellar ataxia type 17), which is characterized by ataxia, dystonia, parkinsonism, and chorea.
TBP is the core protein of the transcription factor IID complex, the multiprotein DNA-binding factor that coordinates activities necessary for initiation of transcription by RNA polymerase II.
The TATA-binding protein (TBP) is believed to function as an essential factor of the general transcription machinery and to be involved in transcription by all three eukaryotic RNA polymerases (pol I, II, and III). TBP specifically binds to TATA element at the promoter region and interacts with numerous transcription factors, including TBP-associated factors (TAFs), activators, and some tumor suppressor proteins.

Postać fizyczna

Clear and colorless frozen liquid solution

Uwaga dotycząca przygotowania

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number
050115116

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

A unified nomenclature for TATA box binding protein (TBP)-associated factors (TAFs) involved in RNA polymerase II transcription.
Tora L
Genes & Development, 16(6), 673-675 (2002)
The Pathogenic Role of Low Range Repeats in SCA17.
Shin JH, et al.
PLoS ONE, 10(8) (2015)
Crystal structure of a human TATA box-binding protein/TATA element complex.
Nikolov DB, et al.
Proceedings of the National Academy of Sciences of the USA, 93(10), 4862-4867 (1996)
Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies.
A Hoffmann et al.
Nucleic acids research, 19(22), 6337-6338 (1991-11-25)
M Horikoshi et al.
Cell, 54(7), 1033-1042 (1988-09-23)
The mammalian activator protein ATF stimulates transcription from the adenovirus E4 promoter by binding to multiple upstream promoter and enhancer elements. DNAase footprint analyses have revealed that there are cooperative interactions between ATF and TFIID (the mammalian TATA factor) when
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