SRE0023

β-Glucuronidase from abalone

Purified, aqueous solution, β-glucuronidase 150,000-250,000 units/mL, β-glucuronidase ≥20,000,000 units/g protein

• Numer EC enzymu: 3.1.2.31 (BRENDA, IUBMB)
• Numer MDL: MFCD00130629
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• Poziom jakości: 200
• Postać: aqueous solution
• β-glucuronidase activity: ≥20,000,000 units/g protein; 150,000-250,000 units/mL
• secondary activity: ≤7,500 units/mL sulfatase
• temp. przechowywania: 2-8°C

Opis

Zastosowanie

Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine.

Działania biochem./fizjol.

β-Glucuronidase (GUSB) is a lysosomal enzyme, that degrades glucuronate-containing glycosaminoglycans. β-glucuronidase, obtained from abalone has the ability to hydrolyze samples, that has high analyte concentrations. Absence of β-glucuronidase results in mucopolysaccharidosis type VII (MPSVII), which leads to lysosomal storage in the brain.

Definicja jednostki

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

Postać fizyczna

Supplied as an aqueous solution containing L-proline, ammonium acetate and sodium azide

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable