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Merck

SAB4600176

Sigma-Aldrich

Anti-Mouse IgG (H+L), highly cross-adsorbed, CF 647 antibody produced in donkey

~2 mg/mL, affinity isolated antibody

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.46

pochodzenie biologiczne

donkey

białko sprzężone

CF 647 conjugate

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

secondary antibodies

klon

polyclonal

Postać

buffered aqueous solution

reaktywność gatunkowa

mouse

stężenie

~2 mg/mL

metody

flow cytometry: 1-10 μg/mL
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect immunofluorescence: 1-10 μg/mL

fluorescencja

λex 650 nm; λem 665 nm

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

Opis ogólny

Immunoglobulin G (IgG) is a glycoprotein antibody with two equivalent light chains and a pair of identical heavy chains. CF 647 is an organic cyanine dye useful in fluorescent microscopy studies. Antibody–CF 647 conjugates are affinity-purified antibodies labeled with a far-red fluorescent dye, CF 647. CF dyes are excellent alternatives to Alexa Fluor dyes for antibody labeling with exceptional brightness, photostability, and/or specificity.

Specyficzność

Binds all mouse IgGs (minimal cross-reaction with bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit and sheep serum proteins)

Immunogen

mouse IgG (H+L)

Działania biochem./fizjol.

Immunoglobulin G (IgG) on pepsin digestion results in fragment crystallizable (Fc), which comprises the H chain constant region. Papain digestion of IgG results in the generation of fragment antigen-binding (Fab) comprising of one complete L chain and a variable and CH1 region of H chain.

Cechy i korzyści

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Postać fizyczna

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 2 mg/mL bovine serum albumin.

Uwaga dotycząca przygotowania

Protect from light.

Informacje prawne

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
ALEXA FLUOR is a trademark of Life Technologies
CF is a trademark of Biotium, Inc.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Blue-conversion of Organic Dyes Produces Artifacts in Multicolor Fluorescence Imaging
Kim DH, et al.
Chemical Science null
Harry W Schroeder et al.
The Journal of allergy and clinical immunology, 125(2 Suppl 2), S41-S52 (2010-03-05)
Immunoglobulins are heterodimeric proteins composed of 2 heavy and 2 light chains. They can be separated functionally into variable domains that bind antigens and constant domains that specify effector functions, such as activation of complement or binding to Fc receptors.
Gestur Vidarsson et al.
Frontiers in immunology, 5, 520-520 (2014-11-05)
Of the five immunoglobulin isotypes, immunoglobulin G (IgG) is most abundant in human serum. The four subclasses, IgG1, IgG2, IgG3, and IgG4, which are highly conserved, differ in their constant region, particularly in their hinges and upper CH2 domains. These

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