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SAB4200725

Sigma-Aldrich

Anti-Splicing Factor SC-35 antibody, Mouse monoclonal

clone SC-35, purified from hybridoma cell culture

Synonim(y):

Anti-PR264, Anti-SFRS2, Anti-SFRS2A, Anti-SRp30b, Anti-Splicing component 35 kDa (SC35), Anti-serine/arginine-rich splicing factor 2 (SRSF2)

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.43

pochodzenie biologiczne

mouse

forma przeciwciała

purified from hybridoma cell culture

rodzaj przeciwciała

primary antibodies

klon

SC-35, monoclonal

Formularz

buffered aqueous solution

masa cząsteczkowa

~35 kDa

reaktywność gatunkowa

human, mouse, quail, hamster, frog, porcine, rat

stężenie

~1.0 mg/mL

metody

ELISA: suitable
dot blot: suitable
immunoblotting: suitable
immunofluorescence: 0.125-0.25 μg/mL using HeLa cells
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

numer dostępu UniProt

Q01130

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... SRSF2(6427)

Opis ogólny

Anti-Splicing Factor SC-35 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the SC-35 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a RBF-DHJ mouse immunized with partially purified spliceosomes prepared from HeLa cells. Splicing Factor SC-35, also known as serine/arginine rich splicing factor 2 (SRSF2) belongs to the family of of serine/arginine-rich (SR) proteins.

Immunogen

partially purified spliceosomes prepared from HeLa cells

Zastosowanie

Anti-Splicing Factor SC-35 antibody has been used in
  • dot blot
  • enzyme linked immunosorbent assay (ELISA)
  • immunofluorescence
  • immunohistochemistry
  • immunoblotting
  • immunoprecipitation

Działania biochem./fizjol.

Splicing Factor SC-35 are critical regulators of constitutive and alternative pre-mRNA splicing. It also controls cellular apoptosis in response to genotoxic stress. Serine/arginine protein kinase 1 (SRPK1) specifically phosphorylates SC-35, resulting in p53 suppression and cyclin D1 upregulation. Elevated levels of SC-35 correlate with aggressive phenotype in several cancer types including lung squamous cell carcinoma and lung adenocarcinoma. SC-35 has oncogenic function in tumor progression of human papilloma virus (HPV)-infected cells.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number
017M4757
51160804

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Valerie Edmond et al.
The EMBO journal, 30(3), 510-523 (2010-12-16)
SRSF2 is a serine/arginine-rich protein belonging to the family of SR proteins that are crucial regulators of constitutive and alternative pre-mRNA splicing. Although it is well known that phosphorylation inside RS domain controls activity of SR proteins, other post-translational modifications
E2F1 controls alternative splicing pattern of genes involved in apoptosis through upregulation of the splicing factor SC35
Merdzhanova L, et al.
Cell Death and Differentiation, 15(12), 1815-1815 (2008)
D L Spector et al.
The EMBO journal, 10(11), 3467-3481 (1991-11-01)
SC-35 is a non-snRNP spliceosome component that is specifically recognized by the anti-spliceosome monoclonal antibody alpha SC-35. In this paper we provide direct evidence that SC-35 is an essential splicing factor and we examine the immunolocalization of SC-35 by confocal
M Carmo-Fonseca et al.
The EMBO journal, 10(7), 1863-1873 (1991-07-01)
The in vivo distribution of snRNPs has been analysed by microinjecting fluorochrome-labelled antisense probes into the nuclei of live HeLa and 3T3 cells. Probes for U2 and U5 snRNAs specifically label the same discrete nuclear foci while a probe for
X D Fu et al.
Nature, 343(6257), 437-441 (1990-02-01)
A monoclonal antibody raised against mammalian spliceosomes specifically recognizes a non-snRNP factor required for spliceosome assembly. This splicing factor is highly concentrated in discrete regions within the nucleus, in a pattern that is a distinct subset of that seen with
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