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SAB4200720

Sigma-Aldrich

Anti-β-Catenin antibody, Mouse monoclonal

clone 15B8, purified from hybridoma cell culture

Synonim(y):

Anti-CTNNB1, Anti-armadillo

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

200

forma przeciwciała

purified from hybridoma cell culture

rodzaj przeciwciała

primary antibodies

klon

15B8, monoclonal

Postać

buffered aqueous solution

masa cząsteczkowa

antigen ~94 kDa

reaktywność gatunkowa

chicken, mouse, human, rat, monkey, hamster, bovine, canine

stężenie

~1.0 mg/mL

metody

immunoblotting: 0.25-0.5 μg/mL using Madin-Darby bovine kidney (MDBK) cell line extract
immunofluorescence: 5-10 μg/mL using human breast adenocarcinoma MCF7 cells
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

izotyp

IgG1

numer dostępu UniProt

P35222

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)

Opis ogólny

Anti-β-Catenin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 15B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant chicken β-Catenin. β-Catenin, also known as Catenin βfamily.

Immunogen

recombinant chicken ß-Catenin

Zastosowanie

Anti-β-Catenin antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry
  • immunoprecipitation

Działania biochem./fizjol.

β-Catenin plays a dual function in the cell including cell-cell adhesion and as a component of the Wnt (wingless) signaling pathway. In adherens junctions, β-Catenin associates with the cytoplasmic domain of the cadherin family adhesion receptors, anchoring them to the actin cytoskeleton and thus acting as structural protein. β-Catenin interacts with T cell factor (TCF)- family transcription factors to activate target genes. Wnt/β-catenin signaling interacts with several cellular pathways in context-dependent ways, including JakSTAT (Janus kinase−Signal transducer and activator of transcription) cytokine, TGFβ (transforming growth factor−β), DNA damage and the Hippo signaling pathways. Furthermore, β-Catenin is involved in embryonic morphogenesis and tumorigenesis and is tightly regulated by phosphorylation and further degradation by the ubiquitin-proteasome system.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Beyond canonical: the Wnt and beta-catenin story
Moon RT and Gough NR
Science Signaling, 9(422), eg5-eg5 (2016)
Kiyomi Tsuji-Tamura et al.
FEBS letters, 596(13), 1686-1699 (2022-04-02)
Blood vessels normally remain stable over the long term. However, in atherosclerosis, vascular cells leave the quiescent state and enter an activated state. Here, we investigated the factors that trigger the breakage of the quiescent state by screening growth factors
Thomas Andl et al.
Development (Cambridge, England), 131(10), 2257-2268 (2004-04-23)
Bone morphogenetic protein (BMP) signaling is thought to perform multiple functions in the regulation of skin appendage morphogenesis and the postnatal growth of hair follicles. However, definitive genetic evidence for these roles has been lacking. Here, we show that Cre-mediated
E Sadot et al.
Oncogene, 19(16), 1992-2001 (2000-05-10)
Beta-catenin and plakoglobin are closely related armadillo family proteins with shared and distinct properties; Both are associated with cadherins in actin-containing adherens junctions. Plakoglobin is also found in desmosomes where it anchors intermediate filaments to the desmosomal plaques. Beta-catenin, on

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