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Merck

SAB4200025

Sigma-Aldrich

Anti-VPS4 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonim(y):

Anti-Vacuolar protein sorting 4

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

rabbit

białko sprzężone

unconjugated

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

primary antibodies

klon

polyclonal

Postać

buffered aqueous solution

masa cząsteczkowa

antigen ~50 kDa

reaktywność gatunkowa

rat, human, mouse

stężenie

~1.0 mg/mL

metody

immunoprecipitation (IP): 2-4 μg using lysates of human HeLa cells
western blot: 1-2 μg/mL using whole extracts of mouse 3T3 or rat NRK cells

numer dostępu UniProt

Zastosowanie

research pathology

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... VPS4A(27183)
mouse ... Vps4a(116733)
rat ... Vps4a(246772)

Opis ogólny

Vacuolar protein sorting-associated protein 4 (Vps4) is a member of the AAA protein family (ATPases associated with diverse cellular activities). Vps4 exists as two orthologs in humans (VPS4A and VPS4B). The gene encoding Vps4A is mapped to chromosome 16q22, while the gene for Vps4B resides on chromosome 18q21-q22.

Specyficzność

Anti-VPS4 recognizes human, mouse, and rat VPS4A and VPS4B.

Zastosowanie

Anti-VPS4 antibody has been used in
  • immunoprecipitation
  • immunofluorescence Confocal Microscopy
  • immunohistochemistry
  • western blotting

Działania biochem./fizjol.

Vacuolar protein sorting-associated protein 4 (VPS4) mediates endosomal membrane protein sorting, retroviral budding and cytokinesis by recognizing membrane-associated ESCRT-III assemblies and catalyzing their disassembly.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Przechowywanie i stabilność

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

ANCHR mediates Aurora-B-dependent abscission checkpoint control through retention of VPS4
Thoresen SB, et al.
Nature Cell Biology, 16(6), 550-560 (2014)
The AAA-ATPase VPS4 regulates extracellular secretion and lysosomal targeting of alpha-synuclein
Hasegawa T, et al.
PLoS ONE, 6(12), e29460-e29460 (2011)
VPS4 is a dynamic component of the centrosome that regulates centrosome localization of $\gamma$-tubulin, centriolar satellite stability and ciliogenesis
Ott C, et al.
Scientific reports, 8(1), 3353-3353 (2018)
Lauren R Kett et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(14), 5724-5742 (2015-04-10)
Accumulating evidence from genetic and biochemical studies implicates dysfunction of the autophagic-lysosomal pathway as a key feature in the pathogenesis of Parkinson's disease (PD). Most studies have focused on accumulation of neurotoxic α-synuclein secondary to defects in autophagy as the
Aiqin Hu et al.
BMC genetics, 20(1), 7-7 (2019-01-13)
Vacuolar protein sorting-associated protein 4B (VPS4B) is a member of the ATP enzyme AAA protein family, and is mainly involved in protein degradation and cell membrane fusion. Recently, a dominant mutation in this gene was identified in human dentin dysplasia

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