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S9194

Sigma-Aldrich

SYBR® Green JumpStart Taq ReadyMix for High Throughput qPCR

SYBR® Green qPCR reagent, passive reference dye included

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About This Item

Kod UNSPSC:
41106300
NACRES:
NA.55

Poziom jakości

Postać

liquid

zastosowanie

sufficient for 20 reactions
sufficient for 2000 reactions
sufficient for 400 reactions

Właściwości

dNTPs included
hotstart

stężenie

1.25 units/reaction (50 μL reaction volume)

metody

qPCR: suitable

kolor

colorless

moc wejściowa

purified DNA

kompatybilność

for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus

metoda wykrywania

SYBR® Green

Warunki transportu

wet ice

temp. przechowywania

−20°C

Opis ogólny

SYBR® Green JumpStart Taq ReadyMix is a ready-to-use 2X master mix that contains SYBR® Green I, JumpStart Taq DNA polymerase, 99% pure deoxynucleotides (dNTPs), and reaction buffer making it the ideal solution for performing high-throughput quantitative PCR. SYBR® Green I dye binds to and detects any dsDNA generated during amplification. The JumpStart Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.

Zastosowanie

SYBR® Green JumpStart Taq ReadyMix for High Throughput qPCR has been used for the amplification and quantification of transcripts to analyze gene expression in 2-step quantitative reverse transcription PCR (RT-qPCR). It has also been used for quantitative polymerase chain reaction (qPCR) of reverse-transcribed cDNA.

Cechy i korzyści

  • The master mix allows consistency and reproducibility from one reaction to the next
  • Reduced set-up time as compared to manual or wax Hot Start methods
  • Reduced primer dimers
  • Allows for room temperature set-up; Ideal for high throughput, quantitative PCR application
  • SYBR® Green I dye binds to double-stranded DNA and is ideal for quantifying any DNA sequence. Detection is monitored by measuring the increase in fluorescence throughout cycling.
  • JumpStart Taq DNA polymerase prevents amplification of non-specific products while increasing target yield.
  • Internal Reference Dye is provided for reaction normalization. Maximum excitation and emission is 586 nm and 605 nm, respectively.
  • SYBR Green JumpStart Taq ReadyMix reduces preparation time and the risk of contamination from multiple pipetting steps.

Opakowanie

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
400RXN is packaged as 1 X 10 mL
2000RXN is packaged as 1 X 50 mL

Inne uwagi

SYBR Green ReadyMix for High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq and SYBR Green I in an easy-to-use ReadyMix solution that incorporates ROX dye for ABI and other real time instrument applications. The ReadyMix includes a detection fluor, internal standard and reagents for PCR making it the ideal solution for performing high-throughput quantitative PCR.

Informacje prawne

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
This page may contain text that has been machine translated.

Piktogramy

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Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Narcisa Muresu et al.
International journal of environmental research and public health, 17(12) (2020-06-27)
Objectives: Anal cancer is a rare disease. However, its incidence is increasing in some population groups. Infection caused by Human Papillomavirus (HPV) is strongly associated with the risk of anal cancer, whose variability depends on samples, histology, and HPV detection
Sambrook, J., and Russell, D.W.
Molecular Cloning: A Laboratory Manual null
Gene resistance to transcriptional reprogramming following nuclear transfer is directly mediated by multiple chromatin-repressive pathways
<BIG><BIG>Jullien J, et al.</BIG></BIG>
Molecular Cell, 873-884 (2017)
Gene resistance to transcriptional reprogramming following nuclear transfer is directly mediated by multiple chromatin-repressive pathways
Jullien J, et al.
Molecular Cell, 65(5), 873-884 (2017)
Shin-Heng Chiou et al.
Cancer discovery, 7(10), 1184-1199 (2017-08-10)
Pancreatic ductal adenocarcinoma (PDAC) is one of the most metastatic and deadly cancers. Despite the clinical significance of metastatic spread, our understanding of molecular mechanisms that drive PDAC metastatic ability remains limited. By generating a genetically engineered mouse model of

Produkty

Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer

Reakcja łańcuchowa polimerazy jest jedną z najczęściej stosowanych technik w biologii molekularnej. Proces PCR składa się z trzech głównych etapów: denaturacji, wygrzewania i wydłużania.

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

Protokoły

A protocol that can be used as a basic template for qPCR incorporating SYBR Green I DNA binding dye that is amenable to modification and applicable for use as validation for a set of primers.

Nasz protokół SYBR Green qPCR to metoda zaprojektowana do dokładnego wykrywania ilościowego ekspresji genów i reakcji RT-PCR

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

Powiązane treści

RT-qPCR wykrywa określone cele z zastosowaniami w ekspresji genów i wykrywaniu patogenów.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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