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KCQS03

Sigma-Aldrich

KiCqStart® SYBR® Green qPCR ReadyMix™

iQ™, with fluorescein for Bio-Rad systems

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About This Item

Kod UNSPSC:
41106300
NACRES:
NA.55

Postać

liquid

zastosowanie

sufficient for 1250 reactions
 sufficient for 250 reactions
 sufficient for 5000 reactions

Właściwości

dNTPs included
hotstart

warunki przechowywania

protect from light

metody

qPCR: suitable

kolor

colorless

moc wejściowa

purified DNA

kompatybilność

for use with Bio-Rad MyiQ
for use with Bio-Rad iCycler iQ
for use with Bio-Rad iQ 5

metoda wykrywania

SYBR® Green

Warunki transportu

dry ice

temp. przechowywania

−20°C

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Opis ogólny

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.

Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.

Zastosowanie

KiCqStart® SYBR® Green qPCR ReadyMix™ has been used for the qPCR analysis of 16S rRNA.
Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system.
PCR applications:
  • Gene expression
  • DNA quantification
  • CHiP

Cechy i korzyści

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required

Komponenty

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, 20 nM fluorescein, and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Inne uwagi

Storage Conditions:
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.

Informacje prawne

KiCqStart is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
iQ is a trademark of Bio-Rad Laboratories, Inc.
This page may contain text that has been machine translated.

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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Himanshu Kumar Khuntia et al.
SN applied sciences, 2(8), 1320-1320 (2020-08-25)
This research aims to determine the presence of antibiotic-resistant genes (ARG) in anaerobic biofilm reactors (ABR) fed with household chemical products (HCP) such as laundry detergents and handwash without any influx of antibiotics. The ABR comprised a three-chamber design with
Jackson T Sparks et al.
Insect biochemistry and molecular biology, 43(12), 1161-1171 (2013-10-26)
The yellow-fever mosquito, Aedes aegypti, infects a growing number of people every year with dengue, yellow fever and chikungunya viruses. Contact chemoreception in mosquitoes influences a number of behaviors including host-selection, oviposition and feeding. While these behaviors are in many
Jackson T Sparks et al.
Insect biochemistry and molecular biology, 48, 8-16 (2014-03-04)
The yellow-fever mosquito Aedes aegypti is a major vector of human diseases, such as dengue, yellow fever, chikungunya and West Nile viruses. Chemoreceptor organs on the labella and tarsi are involved in human host evaluation and thus serve as potential
Glade Dlott et al.
Journal of microbiological methods, 115, 112-120 (2015-06-10)
We tested a method of estimating the activity of detectable individual bacterial and archaeal OTUs within a community by calculating ratios of absolute 16S rRNA to rDNA copy numbers. We investigated phylogenetically coherent patterns of activity among soil prokaryotes in
Stephanie Yarwood et al.
Microbial ecology, 69(2), 383-394 (2014-11-06)
The process of pedogenesis and the development of biological communities during primary succession begin on recently exposed mineral surfaces. Following 30 years of surface exposure of reclaimed surface mining sites (Appalachian Mountains, USA), it was hypothesized that microbial communities would
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Produkty

PCR/qPCR Data Analysis

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

Analiza danych PCR/qPCR

Po zakończeniu tradycyjnego PCR, analiza danych PCR/qPCR jest przeprowadzana przez rozdzielczość w żelu agarozowym lub, ostatnio, przez kapilarę.

PCR Assay Optimization and Validation

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Optymalizacja i walidacja testów PCR

Przewodnik po teście PCR prowadzi przez walidację starterów i inne czynniki optymalizacji testu, aby zapewnić wysoką czułość i specyficzność dla optymalnej kwantyfikacji DNA / RNA.

Protokoły

KiCqStart™ Universal SYBR® Green qPCR Protocol

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

Protokół wyboru genów referencyjnych qPCR

Analiza danych ekspresji genów wymaga stabilnego odniesienia lub kontroli obciążenia. Odniesieniem jest zwykle jeden lub więcej genów referencyjnych.

Protokół określania wydajności qPCR

Optymalizacja warunków qPCR jest ważna dla opracowania solidnego testu. Dwa główne podejścia to optymalizacja stężenia starterów i/lub temperatury annealingu.

Primer Optimization Protocol Using Temperature Gradient

Gradient PCR for assay optimization is to determine the optimum annealing temperature (Ta) of the primers by testing identical reactions containing a fixed primer concentration, across a range of annealing temperatures.

Zobacz wszystko

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