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S3077

Sigma-Aldrich

3dGRO® Spheroid Medium

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About This Item

Kod UNSPSC:
12352207

opakowanie

pkg of 250 mL (basal medium)

Warunki transportu

wet ice

temp. przechowywania

2-8°C

Opis ogólny

Cancer stem cell (CSCs) are defined as a small subset of cells within a tumor with the ability to self-renew and often drive tumor progression and recurrence after chemotherapy treatment. Therefore, development of specific therapies targeted at CSCs holds hope for improvement of survival and quality of life of cancer patients, especially for patients with metastatic disease.
Traditionally, cancer stem cells have been isolated from cancer cell lines and tumor biopsies and grown in undefined serum containing media using 3-dimensional cell cultures. Using these undefined culture conditions leads to experimental variability and suboptimal cell growth.
3dGRO® Spheroid Medium is a new chemically defined and serum-free cancer stem cell media that supports the expansion of CSC′s in 3D spheroid cell cultures. The media supports serial passaging of cancer cell lines leading to an overall enrichment of CSC populations.

Cechy i korzyści

  • Chemically defined, Serum-free formulation.
  • High tumorsphere formation efficiency.
  • Allows extended serial tumorsphere passaging (>10).
  • Proven to enrich CSCs from numerous cancer cell lines.

Przechowywanie i stabilność

Upon receipt, the basal medium can be stored at 4° C and the vial of supplement can be stored at -20° C stably until the expiration date.

Before first use, the the entire contents of the supplement vial should be added to the basal medium. The shelf life of the complete medium after addition of the supplement is 6 weeks when stored at 4° C. It is recommended to aliquot and prewarm only the required amount of complete medium for each use and to continue to store the remainder at 4° C.

Inne uwagi

Cell Lines tested with 3dGRO® Spheroid Medium:
MCF-7, A-431, Panc-1, LNCaP, HT-29, A-549, U-87 MG, HT1080, E006AA

Informacje prawne

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
This page may contain text that has been machine translated.

Kod klasy składowania

10 - Combustible liquids

Certyfikaty analizy (CoA)

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Produkty

Cancer Stem Cell Proliferation in Human Prostate and Breast Cancer Cell Lines Utilizing a New Defined Serum-Free 3D Spheroid Media

Serum-free defined cancer stem cell media used to grow and expand cancer cells in 3D tumorsphere aggregates.

Narzędzia i techniki hodowli komórek 3D

Przegląd hodowli komórek 3D. Dowiedz się więcej o hodowli komórek 2D i 3D, zaletach hodowli komórek 3D i technikach dostępnych do tworzenia modeli komórek 3D

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Protokoły

Cancer Stem Cell Tumorsphere Formation Protocol

Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.

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