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Merck

S1629

Sigma-Aldrich

Sulfatase from Aerobacter aerogenes

Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL

Synonim(y):

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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About This Item

Numer CAS:
Numer EC enzymu:
Numer MDL:
Kod UNSPSC:
12352204
NACRES:
NA.54

typ

Type VI

Poziom jakości

Postać

buffered aqueous glycerol solution

aktywność właściwa

2-5 units/mg protein (biuret)

masa cząsteczkowa

~41 kDa

stężenie

10-20 units/mL

obecność zanieczyszczeń

β-Glucuronidase ≤10 U/mL

Warunki transportu

wet ice

temp. przechowywania

−20°C

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Opis ogólny

Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.

Zastosowanie

Sulfatase from Aerobacter aerogenes has been used:
  • as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses
  • in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1
  • to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides

Działania biochem./fizjol.

Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis. Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates. Sulfatases find application in industry and agriculture.

Definicja jednostki

One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 °C.

Postać fizyczna

Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.
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substrat

Numer produktu
Opis
Cennik

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Melanie Glauser et al.
Clinica chimica acta; international journal of clinical chemistry, 430, 125-128 (2014-01-15)
Total (i.e. free+sulfated) metanephrines in plasma is a biomarker for the diagnosis of pheochromocytoma/paraganglioma. Sulfated metanephrines must be completely deconjugated by perchloric acid hydrolysis or sulfatase treatment prior to analytical measurement to enable quantification by current techniques. In this report
T Saidha et al.
Archives of biochemistry and biophysics, 272(1), 237-244 (1989-07-01)
Mitochondria that have been purified from cells of light-grown wild-type Euglena gracilis Klebs var. bacillaris Cori or dark-grown mutant W10BSmL and incubated with 35SO4(2-) and ATP accumulate a labeled compound in the surrounding medium. This compound is also labeled when
Toshiyuki Nakamura et al.
Bioscience, biotechnology, and biochemistry, 75(8), 1506-1510 (2011-08-09)
β-Glucuronidase and sulfatase are the major deconjugating enzymes used in the cleavage of the glucuronate and sulfate moieties, respectively, from certain conjugated food factors including polyphenols. In the present study, we found that compounds having the same molecular weights as
Bioluminescent probes of sulfatase activity.
Jason S Rush et al.
Chembiochem : a European journal of chemical biology, 11(15), 2096-2099 (2010-09-28)
Toyokazu Miura et al.
Bioscience, biotechnology, and biochemistry, 70(6), 1509-1512 (2006-06-24)
Arylsulfatase activity was detected in a bacterial strain, Citrobacter braakii 69-b, isolated from soil by enrichment cultivation using porcine gastric mucin. The production of arylsulfatase was derepressed markedly in a synthetic medium by the addition of tyramine. The purified enzyme

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