RDD002

HEPES

≥99.5%, crystalline powder, anhydrous, Redi-Dri^™

• Synonim(y): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Wzór empiryczny (zapis Hilla): C₈H₁₈N₂O₄S
• Numer CAS: 7365-45-9
• Masa cząsteczkowa: 238.30
• Beilstein: 883043
• Numer WE: 230-907-9
• Numer MDL: MFCD00006158
• Kod UNSPSC: 12161700
• Identyfikator substancji w PubChem: 329824215
• NACRES: NA.25

Właściwości

• product name: HEPES, anhydrous, free-flowing, Redi-Dri^™, ≥99.5%
• klasa czystości: anhydrous
• Poziom jakości: 100
• linia produktu: Redi-Dri^™
• Próba: ≥99.5%
• Postać: crystalline powder
• jakość: free-flowing
• pH: 5.0-6.5 (25 °C, 238 g/L)
• przydatny zakres pH: 6.8-8.2
• pKa (25°C): 7.5
• rozpuszczalność: water: 500 mg/mL, clear, colorless
• ciąg SMILES: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• Klucz InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Opis

Opis ogólny

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Zastosowanie

HEPES has been used:

• In the novel vesicle-budding protocol as a component in stock solution and buffer solutions
• As a component in intracellular solution for the preparation of brain-slice recording
• As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification
• To maintain the pH at 7 in thermal denaturation assay of purified protein
• In isocratic elution of protein using gel filtration
• Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy

Informacje prawne

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 13 - Non Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable