391340

HEPES

≥99% (alkalimetric), solid, pH 6.8-8.2, Molecular Biology

• Synonim(y): HEPES, N-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Wzór empiryczny (zapis Hilla): C₈H₁₈N₂O₄S
• Numer CAS: 7365-45-9
• Masa cząsteczkowa: 238.30
• Beilstein: 883043
• Numer MDL: MFCD00006158
• Kod UNSPSC: 12161704
• NACRES: NA.25

Właściwości

• product name: HEPES, Free Acid, Molecular Biology Grade, A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.
• klasa czystości: Molecular Biology
• Poziom jakości: 100
• Próba: ≥99% (alkalimetric)
• Postać: solid
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze
• kolor: white
• przydatny zakres pH: 6.8-8.2
• pKa (25°C): 7.5
• rozpuszczalność: water: 400 mg/mL; aqueous base: soluble
• ślady kationów: heavy metals: ≤0.0005%
• obecność zanieczyszczeń: DNase, RNase, and protease, none detected
• Warunki transportu: ambient
• temp. przechowywania: 15-25°C
• ciąg SMILES: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• Klucz InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Opis

Opis ogólny

A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pK_a of 7.48 at 25°C.

Zastosowanie

• Fluorescent labeling of the root cap cells with the bioactive NBD-S chemical probe based on the cellulose biosynthesis inhibition herbicides: This innovative study demonstrates the application of molecular biology grade HEPES in the formulation of bioactive probes for the fluorescent labeling of root cap cells. Using cellulose biosynthesis inhibition herbicides as a model system, this research provides valuable insights into plant biology, aiding in the understanding of plant cell wall structures and functions. The use of HEPES helps maintain the physiological pH during the experiments, ensuring the stability and effectiveness of the chemical probes used (Tamogami S et al., 2021).

Ostrzeżenie

Toxicity: Standard Handling (A)

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 13 - Non Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable