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Merck

R5886

SAFC

RPMI-1640 Medium

HEPES modification, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonim(y):

Roswell Park Memorial Institute 1640 medium

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About This Item

Numer MDL:
Kod UNSPSC:
12352207
NACRES:
NA.75

Nazwa produktu

RPMI-1640 Medium, HEPES Modification, With 25 mM HEPES, without L-glutamine., liquid, sterile-filtered, suitable for cell culture

sterylność

sterile-filtered

Formularz

liquid

metody

cell culture | mammalian: suitable

zanieczyszczenia

endotoxin, tested

pH

>7.2

komponenty

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes

Warunki transportu

ambient

temp. przechowywania

2-8°C

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Opis ogólny

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good′s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.

Zastosowanie

RPMI-1640 Medium has been used:
  • to culture human lymphoblastoid cell line cells
  • prostate cancer cells
  • monocytes from human blood

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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Stephanie Puukila et al.
International journal of radiation biology, 95(8), 1072-1084 (2019-05-30)
Purpose: Diagnostic radiation is an important part of patient care in the Intensive Care Unit; however, there is little data on the acute effects of exposure to these doses. We investigated pulmonary and splenic response 30 minutes, 4 hours or 24 hours after
Marga J Bouma et al.
Stem cell research, 41, 101654-101654 (2019-11-18)
Lymphoblast cells from four individuals of a family of two genetically unrelated parents and their monozygotic twins were used to generate integration-free human induced pluripotent stem cells (hiPSCs). Reprogramming factors were delivered by co-electroporation of three episomal-based plasmids expressing OCT3/4
Katrin Kleinmanns et al.
EBioMedicine, 56, 102783-102783 (2020-05-27)
The completeness of resection is a key prognostic indicator in patients with ovarian cancer, and the application of tumour-targeted fluorescence image-guided surgery (FIGS) has led to improved detection of peritoneal metastases during cytoreductive surgery. CD24 is highly expressed in ovarian
Iveta Gažová et al.
Frontiers in cell and developmental biology, 8, 498-498 (2020-07-29)
The response of the human acute myeloid leukemia cell line THP-1 to phorbol esters has been widely studied to test candidate leukemia therapies and as a model of cell cycle arrest and monocyte-macrophage differentiation. Here we have employed Cap Analysis
Serena De Lucia et al.
PloS one, 13(1), e0191084-e0191084 (2018-01-18)
Artemisinin resistance is a major threat to malaria control efforts. Resistance is characterized by an increase in the Plasmodium falciparum parasite clearance half-life following treatment with artemisinin-based combination therapies (ACTs) and an increase in the percentage of surviving parasites. The

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