R2655
Anti-Rabbit IgG, Native antibody, Mouse monoclonal
clone RabT-50, purified from hybridoma cell culture
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About This Item
Kod UNSPSC:
12352203
NACRES:
NA.46
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
secondary antibodies
klon
RabT-50, monoclonal
Formularz
buffered aqueous solution
reaktywność gatunkowa
rabbit
spodziewany brak reakcji z
human, rat, chicken, cat, bovine, monkey, goat, pig, horse
stężenie
~1 mg/mL
metody
immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using 10 ng of non-reduced rabbit IgG (Cat. No. I5006) per lane
izotyp
IgG1
Warunki transportu
dry ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
Powiązane kategorie
Opis ogólny
Anti-Rabbit IgG, Native antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma RabT-50 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Immunoglobulin G (IgG) is found abundantly in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy and the light chains are linked at VH and CH1 domain to form the Fragment antigen binding (Fab) arm. The antigen binds to the V regions of the antibody.
This antibody reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG.
Specyficzność
Anti-Rabbit IgG, Native antibody, Mouse monoclonal reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG. It does not recognize human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs.
Immunogen
Enriched fraction of rabbit IgG light chain.
Zastosowanie
Anti-Rabbit IgG, Native antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunocytochemistry
- immunohistochemistry
- enzyme-linked immunosorbent assay (ELISA)
Działania biochem./fizjol.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Przechowywanie i stabilność
For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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