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PE0240
Plant Fractionated Protein Extraction Kit
Suitable for any plant species or tissue
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
41105500
NACRES:
NA.56
Polecane produkty
zastosowanie
sufficient for 20 extractions
temp. przechowywania
−20°C
Opis ogólny
Sigma′s Plant Fractionated Protein Extraction Kit is designed specifically for use in plant bioscience to extract qualitative hydrophilic and hydrophobic proteins samples from any type of plant species or tissue for downstream proteomic applications. The protocol does not require any ultracentrifugation or aqueous polymer two-phase partitioning (APTP). The kit includes five reagents; a plant specific protease inhibitor, a specially formulated reagent to extract hydrophilic proteins and a new chaotropic reagent with increased solubilizing power to extract more hydrophobic proteins. Also included in the kit are reducing reagent Tributylphosphine (TBP) and alkylating reagent, Iodoacetamide. These reagents improve separation during isoelectric focusing and 2-D gel electrophoresis. The protease inhibitor is a mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic, metalloproteases and aminopeptidases. The cocktail contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), bestatin, pepstatinA, E-64, leupeptin, and 1,10-phenanthroline.
Following removal of polyphenolics, tannins and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in a specially formulated reagent to extract hydrophilic proteins. After sequential extractions of hydrophilic proteins, addition of the chaotropic reagent provided extracts hydrophobic membrane bound proteins. Plant debris is pelleted by centrifugation and protein extract solutions are collected. The end results are qualitative fractionated protein samples, ready for downstream proteomic analysis.
Following removal of polyphenolics, tannins and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in a specially formulated reagent to extract hydrophilic proteins. After sequential extractions of hydrophilic proteins, addition of the chaotropic reagent provided extracts hydrophobic membrane bound proteins. Plant debris is pelleted by centrifugation and protein extract solutions are collected. The end results are qualitative fractionated protein samples, ready for downstream proteomic analysis.
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Elementy zestawu są też dostępne oddzielnie
Numer produktu
Opis
Karta charakterystyki
- Plant Protein Extraction Reagent Type 1 1 bottle
- C0356Protein Extraction Reagent Type 4 1 bottleKarta charakterystyki
- P9599Protease Inhibitor Cocktail, for plant cell and tissue extracts, DMSO solution 3 x 1Karta charakterystyki
- T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 3 x 0.5Karta charakterystyki
- A3221Iodoacetamide, Single use vial of 56 mg 3 vial(s)Karta charakterystyki
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
Organy docelowe
Respiratory system
Kod klasy składowania
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certyfikaty analizy (CoA)
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