P8170
Protein G−Peroxidase from Streptococcus sp.
recombinant, expressed in unspecified host, lyophilized powder
Synonim(y):
Protein G−HRP from Streptococcus sp.
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About This Item
Polecane produkty
rekombinowane
expressed in unspecified host
Poziom jakości
białko sprzężone
peroxidase conjugate
Formularz
lyophilized powder
metody
direct ELISA: 1:100,000 using human IgG
temp. przechowywania
−20°C
Opis ogólny
Protein G is a bacterial cell wall protein, which has high affinity for immunoglobulin G (IgG). It is isolated from group G streptococcal strains. Peroxidase from Streptococcus sp. is used to maintain the H2O2 homeostasis in cells. It aids the regulation of ABC Mn2+-permease complex (psaBCA) genes.
Specyficzność
The product binds IgG for most mammalian species (excluding feline) and can be used in ELISA or immunohistochemical formats. Protein was determined to be at 250-350ug per 250ug vial by UV absorbance. Reactive to chicken IgG as well.
Zastosowanie
Protein G- Peroxidase from Streptococcus sp. has been used in indirect ELISA and immunoblotting.
Uwaga dotycząca przygotowania
Labeled with peroxidase type VI by a modification of the procedure of O′Sullivan, M.S., et al., FEBS Lett., 95, 311 (1978) which favors low molecular weight conjugates. Conjugate is purified by gel filtration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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Protokoły
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
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