P8045
Protein G-Biotin from Streptococcus sp.
recombinant, expressed in unspecified host, lyophilized powder
Synonim(y):
Biotinylated Protein G, Protein G Biotin, Protein G-Biotin
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About This Item
Polecane produkty
rekombinowane
expressed in unspecified host
Poziom jakości
białko sprzężone
biotin conjugate
Postać
lyophilized powder
skład
Protein, ~90% biuret
zakres etykietowania
2-4 mol d-biotin per mol protein
metody
direct ELISA: 1:70,000 using human IgG
temp. przechowywania
2-8°C
Opis ogólny
Protein G binds to antibodies reversibly and thus, it is considered in antibody purification. It is a 20 kDa protein.
Protein G is an immunoglobulin (IgG) specific bacterial cell wall protein isolated from Streptococci (group G). Native proteins G consist of two IgG-binding domains and albumin and cell surface binding sites. Biotin is a water soluble vitamin that acts as a coenzyme for carboxylase enzyme reactions. Biotin deficiency results in deregulation of metabolic pathways and various physiological impairments. Biotin deficiency is known to cause birth anomalies indicating its important role in development and differentiation of embryos.
Specyficzność
Binds IgG only from most mammalian species except cat; binds chicken IgG.
Zastosowanie
Protein G-Biotin from Streptococcus sp. has been used during incubation for synergistic immunocapture of antigen which was performed using streptavidin-coated magnetic beads.
Protein G-Biotin from Streptococcus sp. has been used in electrochemical biosensing, for the attachment of antibodies with different specificities.
Opakowanie
Package size based on protein content
Inne uwagi
Truncated - lacks albumin, Fab and membrane binding sites.
Uwaga dotycząca przygotowania
Prepared using biotinamidocaproate N-hydroxysuccinimide which incorporates an aminocaproyl spacer.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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