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Merck

P0497

Sigma-Aldrich

Anti-PMP70 antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

Synonim(y):

Anti-70 kDa Peroxisomal membrane Protein, Anti-ABCD3, Anti-PXMP1

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About This Item

Numer MDL:
Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

rabbit

białko sprzężone

unconjugated

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

primary antibodies

klon

polyclonal

Postać

buffered aqueous solution

masa cząsteczkowa

antigen 70 kDa

reaktywność gatunkowa

human, mouse, rat

rozszerzona walidacja

independent
Learn more about Antibody Enhanced Validation

metody

immunoprecipitation (IP): 1-2 μg using RIPA extract (0.5 mg) from human HepG2 cells
indirect immunofluorescence: 4-8 μg/mL using mouse NIH3T3 cells
western blot (chemiluminescent): 0.5-1 μg/mL using whole extracts of rat PC12 cells or rat kidney extract or rat liver extract

numer dostępu UniProt

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... ABCD3(5825)
mouse ... Abcd3(19299)
rat ... Abcd3(25270)

Powiązane kategorie

Opis ogólny

The 70 kDa peroxisomal membrane protein (PMP70) is a major component of peroxisomal membranes. The peroxisome is a multifunctional single-membrane organelle associated with eukaryotic cells. PMP70 belongs to the ATP binding cassette (ABC) transporter superfamily.

Immunogen

synthetic peptide corresponding to amino acid residues 644-659 of rat PMP70 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in human.

Zastosowanie

Anti-PMP70 antibody produced in rabbit has been used in:
  • immunocytochemistry
  • immunostaining
  • immunofluorescence
  • western blotting
  • immunoprecipitation

Działania biochem./fizjol.

PMP70 participates in the metabolic transport of long and very long fatty acids into peroxisomes. PMP70 interacts and forms a stable complex with the adrenoleukodystrophy protein(ALDP), and also with several other peroxisomal proteins. ATP binding/hydrolysis by PMP70 and ALDL and their phosphorylation are involved in the regulation of fatty acid transport into peroxisomes. Mutations in the PMP70 (PXMP1) gene may cause a subset of Zellweger syndrome, an autosomal recessive disorder and is characterized by defects in import mechanism for peroxisomal matrix enzymes.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

nwg

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

The pestivirus N terminal protease Npro redistributes to mitochondria and peroxisomes suggesting new sites for regulation of IRF3 by Npro
Jefferson M, et al.
Testing, 9(2), e88838-e88838 (2014)
Kyle A Lyman et al.
The Journal of biological chemistry, 292(43), 17718-17730 (2017-09-10)
Tetratricopeptide repeat (TPR) domains are ubiquitous structural motifs that mediate protein-protein interactions. For example, the TPR domains in the peroxisomal import receptor PEX5 enable binding to a range of type 1 peroxisomal targeting signal motifs. A homolog of PEX5, tetratricopeptide
Pioglitazone significantly prevented decreased rate of neural differentiation of mouse embryonic stem cells which was reduced by Pex11beta knock-down
Esmaeili M, et al.
Neuroscience, 312, 35-47 (2016)
Direct membrane association drives mitochondrial fission by the Parkinson disease-associated protein alpha-synuclein
Nakamura K, et al.
Test, 286(23), 20710-20726 (2011)
Mutations in the 70K peroxisomal membrane protein gene in Zellweger syndrome
Gartner J, et al.
Nature Genetics, 1(1), 16-16 (1992)

Produkty

Wirowanie oddziela organelle na podstawie rozmiaru, kształtu i gęstości, ułatwiając frakcjonowanie subkomórkowe w różnych próbkach.

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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