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Merck

O6264

Sigma-Aldrich

Anti-O-GlcNAc Transferase (DM-17) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonim(y):

Anti-O-linked N-Acetylglucosamine Transferase, Anti-OGT

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About This Item

Numer MDL:
Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

rabbit

białko sprzężone

unconjugated

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

primary antibodies

klon

polyclonal

Postać

buffered aqueous solution

masa cząsteczkowa

antigen 110 kDa

reaktywność gatunkowa

rat, mouse, human

metody

immunoprecipitation (IP): 2.5-5.0 μg using lysates of HEK 293-T cells
microarray: suitable
western blot: 1-2 μg/mL using HeLa nuclear extracts

numer dostępu UniProt

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... OGT(8473)
mouse ... Ogt(108155)
rat ... Ogt(26295)

Opis ogólny

OGT (O-linked N-acetylglucosamine (O GlcNAc) transferase) is a highly conserved cellular glucosyltransferase enzyme. It is an abundantly expressed protein. Its C-terminal contains the catalytic site, and the N-terminal contains multiple tetratricopeptide (TRP) repeats which constitute a long flexible scaffold.
OGT (O-linked n-acetylglucosamine (O GlcNAc) transferase) enzyme is an homotrimer consisting of three subunits of 110 kDa each. The gene encoding OGT is mapped to human chromosome Xq13.1.

Immunogen

synthetic peptide corresponding to amino acids 740-756 of human O-GlcNAc transferase, conjugated to KLH via an N-terminal added cysteine residue.

Zastosowanie

Anti-O-GlcNAc Transferase (DM-17) antibody produced in rabbit has also been used for immunoprecipitation.
Anti-O-GlcNAc Transferase (DM-17) antibody produced in rabbit has been used for Western Blotting.

Działania biochem./fizjol.

OGT (O-linked N-acetylglucosamine (O GlcNAc) transferase) is responsible for the O-linked addition of a single N-acetylglucosamine (GlcNAc) moiety to Ser and Thr residues during posttranslational modifications of nuclear and cytoplasmic proteins. It is, therefore, indirectly implicated in intracellular signaling by modulating the activity, stability, localization, and interactions of a variety of proteins. It promotes herpes simplex virus (HSV) replication, as it proteolytically cleave HCF-1 (host cell factor 1) transcriptional regulator responsible for transactivation of the immediate-early genes of HSV. In idiopathic pulmonary arterial hypertension (IPAH), this protein is responsible for alteration in glycosylation and proliferation of pulmonary artery smooth muscle cells (PASMCs).
The O-GlcNAcylation of intracellular proteins can occur on phosphorylation sites, and have been implicated in the regulation of gene transcription, diabetes, and neurological processes. O-GlcNAc modifications of nucleocytoplasmic proteins may serve as a negative feedback system for insulin signaling.(3,4) It is widely accepted that modification of serine and threonine residues by O-GlcNAc transferase is involved in the control of transcription, either by controlling elongation by RNA polymerase II or by targeting transcription factors to promoters. Growing evidences show that O-GlcNAc is involved in repressing transcription. OGT was also shown to interact with an histone deacetylase complex by binding to the correpressor Sin3A. This interaction leads to the repression of transcription after transcription factors and RNA polymerase II are modified by addition of O-GlcNAc. Strikingly, O-GlcNAc modification reversibly inhibits proteosomal function in an ubiquitin independent fashion.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Karim Rafie et al.
Open biology, 7(6) (2017-07-01)
O-linked
Ten-eleven translocation 1 (Tet1) is regulated by O-GlcNAc transferase (Ogt) for target gene repression in mouse embryonic stem cells
Shi F, et al.
Test, jbc-M113 (2013)
Protein O-GlcNAcylation in diabetes and diabetic complications
Ma J and Hart GW
Expert Review of Proteomics, 10(4), 365-380 (2013)
O-GlcNAc modification is an endogenous inhibitor of the proteasome
Zhang F, et al.
Cell, 115(6), 715-725 (2003)
Diverse regulation of protein function by O-GlcNAc: a nuclear and cytoplasmic carbohydrate post-translational modification
Vosseller K, et al.
Current Opinion in Chemical Biology, 6(6), 851-857 (2002)

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