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E5134

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)

Synonim(y):

Sequestrene Na₂, Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2

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About This Item

Wzór empiryczny (zapis Hilla):
C10H14N2Na2O8 · 2H2O
Numer CAS:
6381-92-6
Masa cząsteczkowa:
372.24
Beilstein:
3900609
Numer WE:
205-358-3
Kod UNSPSC:
12352107
Identyfikator substancji w PubChem:
24894605
NACRES:
NA.31

klasa czystości

for molecular biology

Poziom jakości

300

Próba

99.0-101.0% (titration)

Postać

powder

przydatność reakcji

reagent type: chelator

metody

electrophoresis: suitable

zanieczyszczenia

≤0.005% Insolubles
≤0.1% Nitrilotriacetic acid (NTA)

mp

248 °C (dec.) (lit.)

rozpuszczalność

3  M NaOH: 1.6 g/10 mL

ślady kationów

Fe: ≤0.005%
Pb: ≤0.002%

przydatność

suitable for electrophoresis
suitable for molecular biology

obecność zanieczyszczeń

DNase, RNase, NICKase and protease, none detected

temp. przechowywania

room temp

ciąg SMILES

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

Klucz InChI

OVBJJZOQPCKUOR-UHFFFAOYSA-L

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Opis ogólny

EDTA disodium salt dehydrate chelates metal ions, forming an octahedral complex with divalent cations. This is useful in molecular biology research to inhibit enzymes dependent on activation by metal ions. In diagnostic assays, it acts as an anticoagulant during blood collection for hematological tests

Zastosowanie

EDTA disodium salt has been used in research:
  • as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more
  • an anticoagulant for whole blood collection
  • a stabilizer solution for freezing cell culture samples
  • in combination with PBS and trypsin, to trypsinize cells
  • for phytoextraction of heavy metals in polluted soils
Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Cechy i korzyści

  • Molecular Biology grade, suitable for many molecular biology applications
  • Free of endonucleases DNase, RNase, Nickase, and Protease

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
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produkt podobny

Numer produktu
Opis
Cennik

Piktogramy

Exclamation markHealth hazard
GHS07,GHS08

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H332 - H373

Zwroty wskazujące środki ostrożności

P260 - P271 - P304 + P340 + P312 - P314 - P501

Klasyfikacja zagrożeń

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

Organy docelowe

Respiratory Tract

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Expression of ABCG2 and Bmi-1 in oral potentially malignant lesions and oral squamous cell carcinoma.
Dalley AJ, et al.
Cancer Medicine, 3(2), 273-283 (2014)
External quality assessment of methylmalonic acid and total homocysteine.
M?ller J, et al.
Clinical Chemistry, 45(9), 1536-1542 (1999)
Erwin G Zoetendal et al.
Nature protocols, 1(2), 954-959 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that consists of numerous uncultured microbes. Therefore, nucleic-acid-based approaches have been introduced to study microbial diversity and activity, and these depend on the proper isolation of DNA, rRNA and mRNA.
Erwin G Zoetendal et al.
Nature protocols, 1(2), 870-873 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that develops in time and space. The most widely used approaches to study microbial diversity and activity are all based on the analysis of nucleic acids, DNA, rRNA and mRNA.
Michi Miura et al.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 28(3-4), 381-393 (2020-10-19)
CUT&RUN is a powerful tool to study protein-DNA interactions in vivo. DNA fragments cleaved by the targeted micrococcal nuclease identify the footprints of DNA-binding proteins on the chromatin. We performed CUT&RUN on human lung carcinoma cell line A549 maintained in
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