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MINI67

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Synonim(y):

Green PKH membrane mini labeling kit

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About This Item

Kod UNSPSC:
12352207
NACRES:
NA.32

opakowanie

pkg of 1 kit

Poziom jakości

producent / nazwa handlowa

Distributed for Phanos Technologies

warunki przechowywania

protect from light

metody

flow cytometry: suitable

fluorescencja

λex 490 nm; λem 502 nm (PKH67 dye)

Zastosowanie

cell analysis
detection

metoda wykrywania

fluorometric

Warunki transportu

ambient

temp. przechowywania

room temp

Opis ogólny

PKH67 kit is for general cell membrane labeling. It has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Zastosowanie

PKH67 green fluorescent cell linker mini kit for general cell membrane labeling has been used to stain:
  • exosomes
  • pericytes
  • UT-7/TPO cells

This kit is for general cell membrane labeling. Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes.

Powiązanie

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Informacje prawne

Distributed for Phanos Technologies, Inc.
CellVue is a registered trademark of Phanos Technologies
This page may contain text that has been machine translated.

Tylko elementy zestawu

Numer produktu
Opis

  • Diluent C 10 mL

  • PKH67 Cell Linker in ethanol 0.1 mL

Piktogramy

FlameExclamation mark

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Eye Irrit. 2 - Flam. Liq. 2

Kod klasy składowania

3 - Flammable liquids

Temperatura zapłonu (°F)

55.4 °F - closed cup

Temperatura zapłonu (°C)

13 °C - closed cup


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

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Produkty

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

A video about how you can use fluorescent cell tracking dyes in combination with flow and image cytometry to study interactions and fates of different cell types in vitro and in vivo.

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