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MIDI67

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Synonim(y):

Green PKH membrane midi labeling kit

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About This Item

Kod UNSPSC:
12352207
NACRES:
NA.32

opakowanie

pkg of 1 kit

Poziom jakości

100

producent / nazwa handlowa

Distributed for Phanos Technologies

warunki przechowywania

protect from light

metody

flow cytometry: suitable

fluorescencja

λex 490 nm; λem 502 nm (PKH67 dye)

Zastosowanie

cell analysis
detection

metoda wykrywania

fluorometric

Warunki transportu

ambient

temp. przechowywania

room temp

Opis ogólny

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Zastosowanie

PKH67 Green Fluorescent Cell Linker Midi Kit has been used for:
  • labeling exosomes  
  • extracellular vesicles (EVs)l
  • normal and sickle red blood cells (RBCs)
  • Raji cells

Powiązanie

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Informacje prawne

CellVue is a registered trademark of Phanos Technologies
This page may contain text that has been machine translated.

Tylko elementy zestawu

Numer produktu
Opis
  • PKH67 Dye 2 x 0.1
  • Diluent C 6 x 10

Piktogramy

FlameExclamation mark
GHS02,GHS07

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H225,H319

Zwroty wskazujące środki ostrożności

P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338

Klasyfikacja zagrożeń

Eye Irrit. 2 - Flam. Liq. 2

Kod klasy składowania

3 - Flammable liquids

Temperatura zapłonu (°F)

57.2 °F - closed cup

Temperatura zapłonu (°C)

14 °C - closed cup

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

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Dongxin Tang et al.
Clinical and translational medicine, 10(4), e139-e139 (2020-09-09)
Cancer stem cells (CSCs) are important factors contributing to tumorigenesis. We examined whether CSCs isolated from colorectal cancer (CRC) cells possess metastatic properties that can be transferred to non-CSCs via the delivery of miR-200c enclosed in extracellular vesicles (EVs). The
Notochordal-cell derived extracellular vesicles exert regenerative effects on canine and human nucleus pulposus cells
Bach F, et al.
Testing, 8(51), 88845-88845 (2017)
Modulation of Sickle Red Blood Cell Adhesion and its Associated Changes in Biomarkers by Sulfated Nonanticoagulant Heparin Derivative
Alshaiban A, et al.
Clinical and Applied Thrombosis/Hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 22, 230-238 (2016)
ADCC Assay Protocol
Fang L and He F
Journal of Virology (2013)
Ioulia Vardaki et al.
Oncotarget, 7(46), 74966-74978 (2016-09-03)
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