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MAK206

Sigma-Aldrich

Fumarase Activity Colorimetric Assay Kit

Sufficient for 100 Colorimetric tests

Synonim(y):

Fumarate Hydratase Activity Colorimetric Assay Kit

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About This Item

Kod UNSPSC:
12352200
NACRES:
NA.84

metoda wykrywania

colorimetric

powiązane choroby

neurological disorders

temp. przechowywania

−20°C

informacje o genach

human ... FH(2271)
mouse ... FH(14193)
rat ... FH(24368)

Opis ogólny

The new Fumarase Assay Kit, MAK521, is now available! Fumarase (Fumarate Hydratase; EC 4.2.1.2) catalyzes the reversible hydration of fumarate to malate in the tricarboxylic acid (Krebs) cycle. The activity of fumarase is important as it produces reducing equivalents that drive the synthesis of ATP and maintains energy balance in aerobic metabolism. A deficiency of fumarase activity results in progressive encephalopathy.

Zastosowanie

Fumarase Activity Colorimetric Assay Kit has been used to determine fumarase (FUM) activity.

Przydatność

Suitable for the detection of Fumarase in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Zasada

The Fumarase Activity Colorimetric Assay Kit provides a simple and high-throughput ready procedure for measuring fumarase activity in a variety of tissues, cells, and isolated mitochondria. Fumarase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of fumarase is the amount of enzyme that generates 1.0 mmole of NADH per minute at pH 9.5 at 37 °C.
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Tylko elementy zestawu

Numer produktu
Opis

  • Fumarase Assay Buffer

  • Fumarase Substrate

  • Fumarase Enzyme Mix

  • Fumarase Developer

  • NADH Standard

  • Fumarase Positive Control

zastąpiony przez

Numer produktu
Opis
Cennik

Piktogramy

Corrosion

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Eye Dam. 1 - Skin Corr. 1B

Kod klasy składowania

8A - Combustible corrosive hazardous materials


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Dieuwertje Augustijn et al.
PloS one, 14(6), e0218219-e0218219 (2019-06-27)
Climate change and the rising food demand provide a need for smart crops that yield more biomass. Recently, two Arabidopsis thaliana mutants with enhanced growth characteristics, VP16-02-003 and the VP16-05-014, were obtained by genome-wide reprogramming of gene expression, which led
Changes in specific protein degradation rates in Arabidopsis thaliana reveal multiple roles of Lon1 in mitochondrial protein homeostasis.
Li L, et al.
The Plant Journal, 89(3), 458-471 (2017)
Lei Li et al.
The Plant journal : for cell and molecular biology, 89(3), 458-471 (2016-10-12)
Mitochondrial Lon1 loss impairs oxidative phosphorylation complexes and TCA enzymes and causes accumulation of specific mitochondrial proteins. Analysis of over 400 mitochondrial protein degradation rates using 15 N labelling showed that 205 were significantly different between wild type (WT) and
Helena Hernández-Cuervo et al.
Frontiers in physiology, 13, 814510-814510 (2022-04-19)
Acute Lung Injury (ALI), characterized by bilateral pulmonary infiltrates that restrict gas exchange, leads to respiratory failure. It is caused by an innate immune response with white blood cell infiltration of the lungs, release of cytokines, an increase in reactive
Ping Li et al.
International journal of molecular sciences, 23(3) (2022-02-16)
The FH gene encodes the fumarate hydratase of the Krebs cycle and functions as a homotetramer to catalyze the hydration of fumarate to malate. Mutations in FH result in uterine leiomyomas, a rare autosomal dominant inherited metabolic disease. However, how

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