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MAK193

Sigma-Aldrich

Citrate Synthase Activity Assay Kit

Sufficient for 100 Colorimetric tests

Synonim(y):

Zestaw testowy syntazy cytrynianowej

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About This Item

Numer WE:
Kod UNSPSC:
12161503
NACRES:
NA.84

metoda wykrywania

colorimetric

powiązane choroby

endocrinological disorders, diabetes; cancer

temp. przechowywania

−20°C

informacje o genach

human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)

Opis ogólny

Citrate Synthase (CS; EC 2.3.3.1) is a mitochondrial enzyme found in all cells capable of oxidative metabolism. It catalyzes the synthesis of citrate from oxaloacetate in the Krebs tricarboxylic acid cycle.1,2 CS is involved in energy production, lipogenesis, and cholesterologenesis, and its activity follows a circadian pattern.

Zastosowanie

Citrate Synthase Activity Assay Kit has been used to measure the activity of the enzyme citrate synthase in samples.

Przydatność

Suitable for the detection of citrate synthase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Zasada

The Citrate Synthase Activity Assay kit provides a simple and direct procedure for measuring the activity of CS in a variety of tissues, cell cultures (adherent or suspension), and purified mitochondria. CS activity is determined using a coupled enzyme reaction, which results in a colorimetric (412 nm) product proportional to the enzymatic activity present. One unit of CS is the amount of enzyme that generates 1.0 mmole CoA per minute at 25 °C and pH 7.2.
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Tylko elementy zestawu

Numer produktu
Opis

  • CS Assay Buffer

  • CS Substrate Mix

  • CS Developer

  • GSH Standard, Reduced

  • CS Positive Control

Piktogramy

Health hazard

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Eye Irrit. 2 - Resp. Sens. 1

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3


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Defective mitochondria have been linked to several critical human diseases such as neurodegenerative disorders, cancers and cardiovascular disease. However, the detailed characterization of mitochondria has remained relatively unexplored, largely due to the lack of effective extraction methods that may sufficiently

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