MAK161

Multidrug Resistance Assay Kit

(Fluorometric MDR Assay)

• Synonim(y): MDR Assay
• Kod UNSPSC: 12161503
• NACRES: NA.84

Właściwości

• zastosowanie: (Fluorometric MDR Assay)
• Zastosowanie: pharmaceutical
• metoda wykrywania: fluorometric
• powiązane choroby: cancer
• temp. przechowywania: −20°C
• informacje o genach: human ... ABCB1(5243) , ABCC2(1244) ; mouse ... ABCC2(12780) ; rat ... abcc2(25303)

Opis

Opis ogólny

Acquired resistance to chemotherapy drugs, multidrug resistance or MDR, is a major contributor to treatment failure for many types of cancers. MDR is typically associated with the increased expression of two ATP-dependent drug efflux pumps, P-Glycoprotein (P-gp or MDR1) and the Multidrug Resistance-Associated Protein (MRP1). These pumps actively expel chemotherapeutic agents, typically hydrophobic amphipathic natural products, from the cytoplasm to exterior of the cell.

This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λ_ex = 490/λ_em = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.

Cechy i korzyści

Compatible with high-throughput handling systems.

Przydatność

This kit is suitable for the screening of MDR pump inhibitors or for identifying cell lines with high MDR activity.

Zasada

This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λ_Ex = 490/λ_Em = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.

This page may contain text that has been machine translated.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids