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Merck

MAK067

Sigma-Aldrich

Malate Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Kod UNSPSC:
12161503
NACRES:
NA.84

zastosowanie

sufficient for 100 colorimetric tests

metoda wykrywania

colorimetric

temp. przechowywania

−20°C

Opis ogólny

L-Malate is a tricarboxylic acid cycle intermediate and a critical component of the malate-aspartate shuttle. The Malate-aspartate shuttle is required for transporting reducing equivalents across the mitochondrial membrane for use in oxidative phosphorylation. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO2. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malate is important in the manufacturing of beer, wine, cheese, and fruits. The Malate Assay kit is an easy and sensitive assay to measure the malate level in a variety of samples.

In this kit, malate is oxidized to generate a product specifically detected by colorimetric (450 nm) assay. Typical sensitivities of detection for this kit are 1-35 nmol of malate.

Zastosowanie

Malate Assay Kit has been used to determine the concentration of malate in samples.

Przydatność

Suitable for the measurement of L(-) Malate level in a variety of samples including tissue, cells and food and beverages (for example beer, wine, cheese and fruit)

Zasada

Malate concentration is determined by an enzymatic assay, which results in a colorimetric (450 nm) product, proportional to the malate present. Typical detection range for this kit is 5–25 nmoles of malate.
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zastąpiony przez

Piktogramy

Corrosion

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Eye Dam. 1 - Skin Corr. 1B

Kod klasy składowania

8A - Combustible corrosive hazardous materials

Klasa zagrożenia wodnego (WGK)

WGK 3


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Odwiedź Bibliotekę dokumentów

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Samuel T Keating et al.
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Liu Z, et al.
Journal of Pineal Research, 64(1), e12455-e12455 (2018)

Produkty

We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.

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