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Key Documents
M6068
Anti-phospho-Myosin Light Chain (pSer19) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
Synonim(y):
Anti-MLC
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About This Item
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
Postać
buffered aqueous glycerol solution
reaktywność gatunkowa
human (predicted), chicken
metody
western blot: 1:1,000 using chicken gizzard myosin II phosphorylated in vitro by PAK
Warunki transportu
dry ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
phosphorylation (pSer19)
informacje o genach
human ... MYL1(4632)
Powiązane kategorie
Opis ogólny
Myosin light chains belongs to the calmodulin (CaM) gene family.
Rabbit polyclonal anti-phospho-Myosin Light Chain (pSer19) antibody detects chicken MLC. Results show that reactivity with the antibody requires phosphorylation of MLC by active kinase, and that only the peptide corresponding to MLC (pSer19) blocks the antibody signal, thereby demonstrating the specificity of the antibody. Human (100% homologous) has not been tested, but is expected to react.
Immunogen
synthetic phosphopeptide derived from the region of chicken MLC that contains serine 19 (serine 20 including the initiating methionine). Human (100% homologous) has not been tested, but is expected to cross-react.
Zastosowanie
Anti-phospho-Myosin Light Chain (pSer19) antibody produced in rabbit has been used in the detection of phosphorylated myosin: in human lung fibroblasts and avian lens cells by immunoblotting by immunohistochemical detection in rabbit aorta samplesby immunofluorescence detection in human submandibular gland tissues
Działania biochem./fizjol.
Myosin light chain is a subunit of the conventional myosins, e.g., myosin II. In smooth muscle and nonmuscle cells, conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however, myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues. Phosphorylation of serine 19, the major phosphorylation site and the preferred site for MLCK, activates myosin motor activity under physiological conditions.
Myosin light chains functions to maintain integrity of myosin protein. Mutations in the myosin light chain is implicated in familial hypertrophic cardiomyopathy.
Postać fizyczna
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (± 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Shuichi Asano et al.
Physiological reports, 5(9) (2017-05-17)
In patients with pulmonary diseases such as idiopathic pulmonary fibrosis and severe acute respiratory distress syndrome, progressive pulmonary fibrosis is caused by dysregulated wound healing via activation of fibroblasts after lung inflammation or severe damage. Migration of fibroblasts toward the
Sara Luck et al.
Molecular vision, 17, 2759-2764 (2011-11-09)
While many studies investigate the cytoskeletal properties of the lens with respect to cataract development, examinations of how these molecular structures interact are few. Myosin light chain kinase (MLCK), actin, and myosin are present on the crystalline lenses of chickens.
The effects of actomyosin disruptors on the mechanical integrity of the avian crystalline lens
Won GJ et al.
Molecular Vision, 21(9), 98-98 (2015)
Myosin light chain kinase inhibitor ML7 improves vascular endothelial dysfunction via tight junction regulation in a rabbit model of atherosclerosis
Cheng X, et al.
Molecular Medicine Reports, 12(3), 4109-4116 (2015)
Myosin light chains: teaching old dogs new tricks
Heissler SM and Sellers JR
Bioarchitecture, 4(6), 169-188 (2014)
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