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Merck

M5670

Sigma-Aldrich

Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit

whole antiserum

Synonim(y):

Anti-MAP Kinase (ERK-1, ERK-2), Anti-Mitogen Activated Protein Kinase

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About This Item

Numer MDL:
Kod UNSPSC:
12352203
NACRES:
NA.44

pochodzenie biologiczne

rabbit

Poziom jakości

białko sprzężone

unconjugated

forma przeciwciała

whole antiserum

rodzaj przeciwciała

primary antibodies

klon

polyclonal

masa cząsteczkowa

antigen ERK-2 42 kDa
antigen, ERK-1 44 kDa

zawiera

15 mM sodium azide

reaktywność gatunkowa

human, rat, mouse

opakowanie

antibody small pack of 25 μL

metody

microarray: suitable
western blot: 1:40,000-1:20,000 using rat brain extract and mouse NIH 3T3 fibroblast extract, respectively

numer dostępu UniProt

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

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Opis ogólny

MAP kinase (MAPK, mitogen-activated protein kinase) is also termed as extracellular regulated protein kinase (ERK). It consists of a family of protein kinases. MAP kinase isoforms are widely expressed in the central nervous system, thymus, spleen, heart, lung, kidney, and in high levels in PC12 cells and in fibroblasts.

Specyficzność

Staining of the 42 kDa and 44 kDa bands is specifically inhibited with MAP kinase peptide (317-339), but not with MAP kinase kinase (MEK) peptide (34-48) corresponding to N-terminal sequence of MEK. Anti-MAPK Kinase (ERK-1, ERK-2) specifically reacts with both phosphorylated and unphosphorylated forms of ERK-1 and ERK-2 (44 and 42 kDa, respectively).

Immunogen

synthetic peptide corresponding to amino acids 317-339 derived from subdomain XI of human MAP kinase (ERK1).

Zastosowanie

Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit has been used in western blot analysis at a minimum dilution of 1:40000 in rat brain extract and at 1:20000 in mouse NIH 3T3 fibroblasts.

Działania biochem./fizjol.

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival. MAPK plays a crucial role in various signal transduction pathways, leading signals of growth factor and G protein coupled receptors to their intracellular targets. MAP kinase regulates several cellular processes including proliferation, differentiatio, cellular morphology and oncogenesis.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

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Zhang X, et al.
International Journal of Molecular Medicine, 35(6), 1747-1754 (2015)
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Duffy DJ, et al.
Molecular Cancer Therapeutics, 13(2), 454-467 (2014)
T T Giang Ho et al.
The Journal of biological chemistry, 283(31), 21588-21598 (2008-06-06)
RhoA plays a significant role in actin stress fibers formation. However, silencing RhoA alone or RhoA and RhoC did not completely suppress the stress fibers suggesting a residual "Rho-like" activity. RhoB, the third member of the Rho subclass, is a
Mitogen-Activated Protein Kinases and Their Role in Radiation Response.
Anupama M and Rajagopal R
Genes & Cancer, 4(9-10), 401-408 (2013)

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