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Kluczowe dokumenty
M5057
MES sodium salt
BioReagent, ≥99% (titration), crystalline
Synonim(y):
2-(N-Morpholino)ethanesulfonic acid sodium salt, 4-Morpholineethanesulfonic acid sodium salt
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About This Item
Wzór empiryczny (zapis Hilla):
C6H12NNaO4S
Numer CAS:
Masa cząsteczkowa:
217.22
Beilstein:
3765682
Numer WE:
Numer MDL:
Kod UNSPSC:
12161700
eCl@ss:
32129211
Identyfikator substancji w PubChem:
NACRES:
NA.25
Polecane produkty
linia produktu
BioReagent
Poziom jakości
Próba
≥99% (titration)
Formularz
crystalline
pH
8.9-10.1 (1% in water)
przydatny zakres pH
5.5-6.7
pKa
6.1
rozpuszczalność
water: 0.5 g/mL, clear, colorless
Zastosowanie
diagnostic assay manufacturing
temp. przechowywania
room temp
ciąg SMILES
[Na+].[O-]S(=O)(=O)CCN1CCOCC1
InChI
1S/C6H13NO4S.Na/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);/q;+1/p-1
Klucz InChI
IRHWMYKYLWNHTL-UHFFFAOYSA-M
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Opis ogólny
MES is one of the so-called "Good" buffers developed for biological applications. This product is tested for trace ions. A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±-1). Alternatively, volumes of equimolar solutions of MES free acid and MES sodium can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.
Zastosowanie
MES sodium salt has been used:
- To achieve the required density and stretching of DNA fibers during melting of agarose plugs containing genomic DNA
- To equilibrate the Balch homogenizer chamber and to prevent sample hydrolysis before sample homogenization
Uwaga dotycząca przygotowania
MES Sodium is soluble in water, giving a clear colourless solution at 25g in 50mL water (approximately 2.3M). The pH of a solution should be between 9 and 10 depending on concentration. Solutions should be stable at 2-8°C for months. Sterilization should be by filteration through 0.2μm filters. Autoclaving is not recommended. If buffers must be nuclease-free, it is best to treat the water first, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.
Inne uwagi
Easily compare specifications for MES sodium salt products with the MES sodium salt specification table.
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Kod klasy składowania
13 - Non Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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