L7261
Lipopolysaccharides from Salmonella enterica serotype typhimurium
purified by trichloroacetic acid extraction
Synonim(y):
LPS
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About This Item
Polecane produkty
pochodzenie biologiczne
Salmonella enterica (Serotype typhimurium)
Poziom jakości
Formularz
lyophilized powder
oczyszczone przez
trichloroacetic acid extraction
zanieczyszczenia
1-10% Protein (Lowry)
kolor
white to faint yellow
rozpuszczalność
water: 0.90-1.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow
Warunki transportu
ambient
temp. przechowywania
2-8°C
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Opis ogólny
This product is TCA extracted from Salmonella typhimurium. The source strain is ATCC 7823. This LPS serotype has been used to induce NOS in rats and guinea pigs.
Zastosowanie
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Działania biochem./fizjol.
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Uwaga dotycząca przygotowania
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Inne uwagi
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
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produkt powiązany
Numer produktu
Opis
Cennik
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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