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L4025
Lyticase from Arthrobacter luteus
lyophilized powder, ≥200 units/mg solid
Synonim(y):
Endohydrolaza (1,3)-β-D-glukanu, Glukohydrolaza 1,3-β-glukanu
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About This Item
Polecane produkty
pochodzenie biologiczne
bacterial (Arthrobacter luteus)
Poziom jakości
Formularz
lyophilized powder
aktywność właściwa
≥200 units/mg solid
metody
cell based assay: suitable
przydatność
suitable for cell lysis
Zastosowanie
diagnostic assay manufacturing
temp. przechowywania
−20°C
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Zastosowanie
Lyticase from Arthrobacter luteus has been used for resuspending cells to extract high-molecular weight DNA for polymerase chain reaction (PCR) fingerprint analyses.
Lyticase from Arthrobacter luteus has been used:
- as a component in spheroplasting buffer to prepare yeast nuclear extracts
- for digestion in sample preparation for β-glucan enzymatic assay using yeast
- to lyse cells for RNA isolation
Działania biochem./fizjol.
Lyticase enzyme is frequently used in fungal research, particularly for species identification using polymerase chain reaction (PCR)-based techniques. It can break down β (1→3) and β (1→4) bonds between glucose units.
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
Lyticase is a lysing enzyme that is used to extract DNA from yeast cells by inducing partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast, which are difficult to disrupt because the cell walls may form capsules or resistant spores. Lyticase contains β-(1→;3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities. It is useful with yeast cells such as e.g. Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.
Definicja jednostki
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
Inne uwagi
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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Protokoły
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
Procedura ta może być stosowana do oznaczania aktywności litykazy przy użyciu drożdży Bakera jako substratu.
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