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Merck

H9664

Sigma-Aldrich

Anti-HMGB1 (HMG1) (N-terminal) antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

Synonim(y):

Anti-Amphoterin, Anti-High mobility group box 1, Anti-High mobility group protein 1

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About This Item

Numer MDL:
Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

rabbit

białko sprzężone

unconjugated

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

primary antibodies

klon

polyclonal

Postać

buffered aqueous solution

masa cząsteczkowa

antigen 25 kDa

reaktywność gatunkowa

human, rat, mouse

rozszerzona walidacja

independent
Learn more about Antibody Enhanced Validation

metody

immunoprecipitation (IP): 10 μg using HEK-293T cell lysates
indirect immunofluorescence: 1-2 μg/mL using paraformaldehyde-Triton fixed PC12 cultured cells.
indirect immunofluorescence: suitable
western blot: 1-2 μg/mL using 3T3 cell lysates

numer dostępu UniProt

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... HMGB1(3146)
mouse ... Hmgb1(15289)
rat ... Hmgb1(25459)

Opis ogólny

Anti-HMGB1 (HMG1) (N-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to human HMGB1 conjugated to KLH. High Mobility Group B (HMGB) protein family includes HMGB1, HMGB2 and HMGB3, that are highly conserved and indistinguishable in their biochemical properties. HMGB1 is a 25 kDa protein of 215 amino acids and consists of two homologous HMG-boxes rich in basic amino acids, and an acidic tail at the carboxy-terminus.
HMGB proteins belong to the High Mobility Group (HMG) family of proteins that contain the HMG-box for binding and changing DNA structures .

Immunogen

synthetic peptide corresponding to amino acids 2-17 of human HMGB1, conjugated to KLH. The corresponding sequence is conserved in mouse and rat.

Zastosowanie

Anti-HMGB1 (HMG1) (N-terminal) antibody is suitable for use in chemiluminescent immunoblot (using mouse heart homogenates) . The antibody can also be used in immunoprecipitation (10 μg using HEK-293T cell lysates), indirect immunofluorescence (1-2 μg/mL using paraformaldehyde-Triton fixed PC12 cultured cells), and western blot (1-2 μg/mL using 3T3 cell lysates).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Działania biochem./fizjol.

High Mobility Group B (HMGB-1 and 2) participate in the regulation of chromatin structure as well as being involved in transcription regulation, DNA repair, recombination, differentiation and extracellular signaling. It shows a defect in transcriptional enhancement of the glucocorticoid receptor, that indicates the important role for HMGB1 in proper transcriptional control by specific transcription factors. Increased expression of HMGB1 is observed in cancer cells.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

HMGB1: endogenous danger signaling
Klune J R, et al.
Molecular Medicine, 14(7-8), 476-484 (2008)
David Frescas et al.
Cell cycle (Georgetown, Tex.), 16(16), 1526-1533 (2017-06-27)
Age-related health decline has been attributed to the accumulation of senescent cells recognized in vivo by p16(Ink4a) expression. The pharmacological elimination of p16(Ink4a)-positive cells from the tissues of mice was shown to extend a healthy lifespan. Here, we describe a
J O Thomas
Biochemical Society transactions, 29(Pt 4), 395-401 (2001-08-11)
HMG1 and 2 (high mobility group proteins 1 and 2; renamed HMGB1 and 2) contain two DNA-binding HMG-box domains (A and B) and a long acidic C-terminal domain. They bind DNA without sequence specificity, but have a high affinity for
Michal Stros
Biochimica et biophysica acta, 1799(1-2), 101-113 (2010-02-04)
HMGB proteins are members of the High Mobility Group (HMG) superfamily, possessing a unique DNA-binding domain, the HMG-box, which can bind non-B-type DNA structures (bent, kinked and unwound) with high affinity, and also distort DNA by bending/looping and unwinding. HMGBs
High-mobility group box 1 and cancer
Tang D, et al.
Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 1799(1-2), 131-140 (2010)

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