GE17-6001-11
Immobiline® DryStrip pH 3-10
Cytiva 17-6001-11, pack of 7 cm
About This Item
Polecane produkty
Postać
solid
Właściwości
wetted part: no
opakowanie
pack of 7 cm
producent / nazwa handlowa
Cytiva 17-6001-11
kolor
Colorless
rozpuszczalność
cold water: soluble (Insoluble in cold and hot water)
Warunki transportu
dry ice
temp. przechowywania
−20°C
Powiązane kategorie
Opis ogólny
Immobiline® DryStrip gels are available in fourteen overlapping pH ranges and five strip lengths (7, 11, 13, 18, and 24 cm) and represent the most comprehensive selection of narrow-, medium-, and wide-range IPG strips available. The five narrow pH range (1 pH unit), 24-cm IPG strips deliver the highest resolution and protein loading capacity available, which results in well separated spots with high protein content after the second-dimension separation.
The pH 3–11 NL strip covers most proteins found in prokaryotic and eukaryotic cells while the narrow-range strips can be used when higher resolution is required. The pH gradients at the extreme ends of the pH scale are nonlinear to distribute the proteins evenly over the gel length to obtain maximal resolution.
Immobiline® DryStrip gels are rehydrated with a matching IPG Buffer prior to use. Samples can be included directly in the rehydration solution for efficient loading of dilute samples or for loading larger amounts of protein. Samples can also be loaded after rehydration. The first-dimension separation is carried out using Ettan™ IPGphor 3 IEF system or Multiphor II flatbed system.
Zastosowanie
Cechy i korzyści
- Maximize resolution, loading capacity, and reproducibility of first-dimension isoelectric focusing (IEF).
- Choose from a comprehensive range of overlapping Immobiline® DryStrip gels that cover narrow (1 pH unit), medium (3–5 pH units), and broad (8 pH units) pH ranges.
- Select the broad pH 3–11 NL gradient Immobiline® DryStrip gel for fast and efficient screening to gain a broad overview of total protein distribution.
- For more detailed studies or when using greater protein loads, choose from four new overlapping strips covering the pH 3 to 11 range.
- Higher resolution can be obtained using narrow-range (1 pH unit) Immobiline® DryStrip gels.
- Use matching IPG buffers for optimized running conditions.
- Use IPG strips directly after reswelling without further manipulation.
- Barcoded strips for convenient sample and batch tracking.
Przechowywanie i stabilność
Komentarz do analizy
Informacje prawne
Kod klasy składowania
13 - Non Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 2
Certyfikaty analizy (CoA)
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Produkty
This page describes troubleshooting first-dimension isoelectric focusing using MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit.
Protokoły
2-D Clean-Up Kit from GE Healthcare is designed to prepare samples that would otherwise produce poor 2-D results.
This page describes troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from GE Healthcare.
This page describes two-dimensional separation of protein samples with GE Healthcare products.
Rehydration Solutions
Powiązane treści
This page shows how to apply equilibrated Immobiline DryStrip gels
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