Wszystkie zdjęcia(1)
Key Documents
G7652
Anti-Mouse IgG (whole molecule)–Gold antibody produced in goat
affinity isolated antibody, aqueous glycerol suspension, 10 nm (colloidal gold)
Synonim(y):
Goat anti-mouse IgG
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Polecane produkty
pochodzenie biologiczne
goat
białko sprzężone
gold conjugate
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
secondary antibodies
klon
polyclonal
Postać
aqueous glycerol suspension
wielkość cząstki
10 nm (colloidal gold)
temp. przechowywania
2-8°C
docelowa modyfikacja potranslacyjna
unmodified
Szukasz podobnych produktów? Odwiedź Przewodnik dotyczący porównywania produktów
Powiązane kategorie
Opis ogólny
Binding activity is determined using a modification of the dot blot assay of Brada and Roth. In this assay a 1 mg/mL solution of mouse IgG is serially diluted in phosphate buffered saline, 1 mL of each dilution is then applied to nitrocellulose paper. Binding activity is defined as the minimum amount of mouse IgG detectable as a visible pink-red spot after a 1 hour incubation with the gold conjugate diluted to an A520 of0.25. Relative order of singlets, doublets, triplets or greater: Singlets>Doublets>Triplets. Clustering data determined by floating the electron microscopy (EM) nickel grid on conjugate diluted 1:100 in Tris buffer, pH 8.0, containing 1% BSA, for 30-60 minutes. The grid is evaluated by EM after drying. One hundred particles are scanned to determine clustering.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (whole molecule)-Gold antibody is specific for mouse IgG.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Immunogold labeling is a technique whereing antibodies, especially secondary antibodies, are labeled with colloidal gold. These gold conjugated antibodies generate electron dense particles that are visable using electron microscopy.
Immunogold labeling is a technique whereing antibodies, especially secondary antibodies, are labeled with colloidal gold. These gold conjugated antibodies generate electron dense particles that are visable using electron microscopy.
Immunogen
Mouse IgG purified from normal mouse serum
Zastosowanie
Anti-Mouse IgG (whole molecule)-Gold antibody is suitable for use in inductively coupled plasma mass spectrometry (ICPMS)-Linked DNA Assay ((1:100). The product can also be used for immunoelectron microscopy (1:20).
Goat polyclonal anti-mouse IgG (whole molecule)–Gold antibody may be used as a primary or secondary antibody that enables the labeling of mouse IgG for detection by electron microscopy.
Postać fizyczna
Colloidal suspension in Tris buffered saline, pH 8.2, with 30% glycerol (v/v), 1% bovine serum albumin (w/v), and 15 mM sodium azide.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Klienci oglądali również te produkty
Daryl E Enstone et al.
Scientific reports, 5, 15074-15074 (2015-10-13)
Hydrophobic protein from soybean (HPS) is present in soybean dust and is an allergen (Gly m 1) that causes asthma in allergic individuals. Past studies have shown that HPS occurs on the seed surface. To determine the microscopic localization of
Arben Merkoçi et al.
Analytical chemistry, 77(19), 6500-6503 (2005-10-01)
Gold nanoparticles modified with anti-mouse IgG have been used to trace oligonucleotides carrying a c-myc peptide. Two strategies, a dot-blot format as well as inductively coupled plasma mass spectrometry (ICPMS) have been used to detect the nanoparticle tracer. For both
Yun Zhang et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 6(1), 67-72 (2002-07-04)
Therapeutic genes are delivered to the nuclear compartment of cancer cells following intravenous administration with a non-immunogenic "artificial virus" gene delivery system that uses receptor-specific monoclonal antibodies (MAb) to navigate the biological barriers between the blood and the nucleus of
Nani Van Gerven et al.
Molecular microbiology, 91(5), 1022-1035 (2014-01-15)
Curli are functional amyloids expressed as fibres on the surface of Enterobacteriaceae. Contrary to the protein misfolding events associated with pathogenic amyloidosis, curli are the result of a dedicated biosynthetic pathway. A specialized transporter in the outer membrane, CsgG, operates
Cassandra Terry et al.
Open biology, 6(5) (2016-06-02)
Mammalian prions are hypothesized to be fibrillar or amyloid forms of prion protein (PrP), but structures observed to date have not been definitively correlated with infectivity and the three-dimensional structure of infectious prions has remained obscure. Recently, we developed novel
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej