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DNTPCA2
CleanAmp™ dNTP
Modified dNTP set for hot-start PCR, 2 μmol of each dNTP
Synonim(y):
Deoxynucleotides, dNTP, dNTP Mix
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About This Item
Polecane produkty
Postać
liquid
jakość
2 μmol of each dNTP
Właściwości
hotstart
stężenie
50 mM (each dNTP)
kolor
colorless
Warunki transportu
dry ice
temp. przechowywania
−20°C
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Opis ogólny
CleanAmp dNTPs, are a product of TriLink BioTechnologies, Inc. CleanAmp dNTPs are modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation. CleanAmp dNTPs are activated by the initial heating step and subsequent denaturing steps in typical hot start PCR cycling conditions. This process limits the amount of activated dNTPs during each cycle of PCR, allowing for more specific and efficient amplification of the desired product and also reducing or completely avoiding mis-priming or primer dimer formation. CleanAmp dNTPs provide a more affordable solution than hot start enzymes for a variety of PCR-based applications.
Zastosowanie
- For PCR amplifications that require reduced non-specific amplification
- For multiplex PCR
- For reduction of primer dimers
- Substitute for dNTPs in any PCR reaction
Opakowanie
2 μmoles: 4 vials:
40 μL dATP at 50 mM in buffered glycine solution
40 μL dCTP at 50 mM in buffered glycine solution
40 μL dTTP at 50 mM in buffered glycine solution
40 μL dGTP at 50 mM in buffered glycine solution
40 μL dATP at 50 mM in buffered glycine solution
40 μL dCTP at 50 mM in buffered glycine solution
40 μL dTTP at 50 mM in buffered glycine solution
40 μL dGTP at 50 mM in buffered glycine solution
Informacje prawne
CleanAmp™ dNTPs are provided to buyer with a non-transferrable right from TriLink BioTechnologies, Inc. to use the purchased CleanAmp™ dNTPs in internal research conducted by the buyer, whether the buyer is an academic, non-profit, or for-profit entity. CleanAmp™ dNTPs are not to be used for human therapeutic or commercial clinical diagnostic use. A license is required for any commercial use of CleanAmp™ dNTPs, regardless of the academic or non-profit status of the using entity. Information about commercial licenses for CleanAmp™ dNTPs may be obtained from TriLink BioTechnologies, Inc. Buyer may not use the CleanAmp™ dNTPs to support the filing of a patent application in any country in the world that contains claims directed to CleanAmp™ dNTPs or uses thereof without the express approval of TriLink BioTechnologies, Inc. Buyer′s right to have and use the CleanAmp™ dNTPs will terminate immediately if you fail to comply with these terms and conditions of this agreement.
CleanAmp is a trademark of TriLink BioTechnologies, Inc.
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Opis
Cennik
Kod klasy składowania
12 - Non Combustible Liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Methods in molecular biology (Clifton, N.J.), 630, 301-318 (2010-03-20)
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Nucleic acids symposium series (2004), (52)(52), 259-260 (2008-09-09)
Several 3'-ether and 3'-ester derivatives of 2'-deoxyribonucleoside 5'-triphosphates (dNTPs) were prepared. These dNTP derivatives were not substrates for DNA polymerase and did not support primer extension at room temperature. However, by short pre-heating to 95 degrees C in PCR buffer
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