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DNTPCA2

Sigma-Aldrich

CleanAmp dNTP

Modified dNTP set for hot-start PCR, 2 μmol of each dNTP

Synonim(y):

Deoxynucleotides, dNTP, dNTP Mix

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About This Item

Kod UNSPSC:
41106305

Postać

liquid

jakość

2 μmol of each dNTP

Właściwości

hotstart

stężenie

50 mM (each dNTP)

kolor

colorless

Warunki transportu

dry ice

temp. przechowywania

−20°C

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Opis ogólny

CleanAmp dNTPs, are a product of TriLink BioTechnologies, Inc. CleanAmp dNTPs are modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation. CleanAmp dNTPs are activated by the initial heating step and subsequent denaturing steps in typical hot start PCR cycling conditions. This process limits the amount of activated dNTPs during each cycle of PCR, allowing for more specific and efficient amplification of the desired product and also reducing or completely avoiding mis-priming or primer dimer formation. CleanAmp dNTPs provide a more affordable solution than hot start enzymes for a variety of PCR-based applications.

Zastosowanie

  • For PCR amplifications that require reduced non-specific amplification
  • For multiplex PCR
  • For reduction of primer dimers
  • Substitute for dNTPs in any PCR reaction

Opakowanie

2 μmoles: 4 vials:
40 μL dATP at 50 mM in buffered glycine solution
40 μL dCTP at 50 mM in buffered glycine solution
40 μL dTTP at 50 mM in buffered glycine solution
40 μL dGTP at 50 mM in buffered glycine solution

Informacje prawne

CleanAmp dNTPs are provided to buyer with a non-transferrable right from TriLink BioTechnologies, Inc. to use the purchased CleanAmp dNTPs in internal research conducted by the buyer, whether the buyer is an academic, non-profit, or for-profit entity. CleanAmp dNTPs are not to be used for human therapeutic or commercial clinical diagnostic use. A license is required for any commercial use of CleanAmp dNTPs, regardless of the academic or non-profit status of the using entity. Information about commercial licenses for CleanAmp dNTPs may be obtained from TriLink BioTechnologies, Inc. Buyer may not use the CleanAmp dNTPs to support the filing of a patent application in any country in the world that contains claims directed to CleanAmp dNTPs or uses thereof without the express approval of TriLink BioTechnologies, Inc. Buyer′s right to have and use the CleanAmp dNTPs will terminate immediately if you fail to comply with these terms and conditions of this agreement.
CleanAmp is a trademark of TriLink BioTechnologies, Inc.
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Opis
Cennik

Kod klasy składowania

12 - Non Combustible Liquids

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Current protocols in molecular biology, Chapter 15, Unit 15-Unit 15 (2009-10-10)
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Methods in molecular biology (Clifton, N.J.), 630, 301-318 (2010-03-20)
Hot Start activation approaches are increasingly being used to improve the performance of PCR. Since the inception of Hot Start as a means of blocking DNA polymerase extension at lower temperatures, a number of approaches have been developed that target
Inna Koukhareva et al.
Nucleic acids symposium series (2004), (52)(52), 259-260 (2008-09-09)
Several 3'-ether and 3'-ester derivatives of 2'-deoxyribonucleoside 5'-triphosphates (dNTPs) were prepared. These dNTP derivatives were not substrates for DNA polymerase and did not support primer extension at room temperature. However, by short pre-heating to 95 degrees C in PCR buffer
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Genome biology, 12(5), R42-R42 (2011-05-17)
Despite its critical role for mammalian gene regulation, the basic structural landscape of chromatin in living cells remains largely unknown within chromosomal territories below the megabase scale. Here, using the 3C-qPCR method, we investigate contact frequencies at high resolution within
Xinglong Xiao et al.
Journal of virological methods, 187(2), 357-361 (2012-11-13)
Nucleic acid testing (NAT) is valuable for screening blood donors for occult hepatitis B virus (HBV) infection and infection during the window period in countries where HBV is endemic, such as China. An "in-house" NAT (Triplex NAT) was developed for

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