Wszystkie zdjęcia(2)
Key Documents
D8935
Nidogen-1 (NID1) human
recombinant, expressed in HEK 293 cells, suitable for cell culture
Synonim(y):
Entactin, NID, NID-1, NID1
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Polecane produkty
rekombinowane
expressed in HEK 293 cells
Próba
95% (SDS-PAGE)
Postać
lyophilized powder
metody
cell culture | mammalian: suitable
temp. przechowywania
−20°C
Opis ogólny
Nidogen-1 belongs to the nidogens family of glycoproteins. This family consists of nidogen-1 and nidogen-2, both of which are sulfated monomeric glycoproteins. Nidogen-1 is ubiquitously present in the synaptic and extra synaptic basal lamina. It is mapped to human chromosome 1q42.3. Nidogen-1 encodes a 139 kDa, three globular domains namely G1, G2 and G3 and has multiple epidermal growth factor repeats. Recombinant human nidogen-1 is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 133.5 kDa (amino acids 29-1247). This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.
Zastosowanie
This recombinant human nidogen-1 product can be used as an attachment factor for cells adherence.
Działania biochem./fizjol.
Nidogen-1 (NID-1) is thought to have an important role in the construction of basement membrane by linking between the networks of laminin and collagen IV. Genetic elimination of the nidogen-1 binding site on the mouse laminin γ1 chain leads to impaired deposition of basement membranes and hampers microvessel maturation. Nidogen 1 and 2 double knockout in mouse fibroblasts led to the loss of all major basal membrane components from the epidermal-collagen interface, and complete abolishment of hemidesmosomes. Supplementation by either recombinant nidogen-1 or -2 improved basal membrane formation. Mutations in NID1and laminin γ1 is implicated in pathogenesis of Dandy-Walker spectrum disorders.
This page may contain text that has been machine translated.
Kod klasy składowania
13 - Non Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Klienci oglądali również te produkty
Mutations in Extracellular Matrix Genes NID1 and LAMC1 Cause Autosomal Dominant D andy-W alker Malformation and Occipital Cephaloceles
Human Mutation, 34(8), 1075-1079 (2013)
Structural elucidation of full-length nidogen and the laminin-nidogen complex in solution
Matrix Biology, 33, 60-67 (2014)
Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease
The Anatomical Record, 300(8), 1371-1390 (2017)
Skin basement membrane: the foundation of epidermal integrity?BM functions and diverse roles of bridging molecules nidogen and perlecan
BioMed Research International, 2013 (2013)
Analysis of nidogen-1/laminin gamma1 interaction by cross-linking, mass spectrometry, and computational modeling reveals multiple binding modes
Testing, 9(11), e112886-e112886 (2014)
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej