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D8764
Deoxyribonuclease II from bovine spleen
Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein
Synonim(y):
DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase
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About This Item
Polecane produkty
pochodzenie biologiczne
bovine spleen
Poziom jakości
typ
Type V
Formularz
essentially salt-free, lyophilized powder
aktywność właściwa
≥1,000 units/mg protein
masa cząsteczkowa
38 kDa
stężenie
≥50% protein
metody
DNA extraction: suitable
tissue culture: suitable
przydatność
suitable for molecular biology
Zastosowanie
cell analysis
obecność zanieczyszczeń
RNase ≤0.1%
temp. przechowywania
−20°C
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Zastosowanie
Deoxyribonuclease II from bovine spleen has been used:
- as a component of digestion mix to isolate immune cells from colon samples
- to digest retinal explants for single-cell suspension preparation
- for isolation of stromal cells from human colon tissue
- in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart
Działania biochem./fizjol.
Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.
Definicja jednostki
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mM
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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