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CPISO

Sigma-Aldrich

Chloroplast Isolation Kit

isolation of intact chloroplasts from leaves

Synonim(y):

Zestaw do izolacji chloroplastów, Zestaw do oczyszczania chloroplastów

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
12352200
NACRES:
NA.32

Poziom jakości

metody

centrifugation: suitable

Warunki transportu

wet ice

temp. przechowywania

2-8°C

Opis ogólny

The Chloroplast Isolation Kit provides an efficient procedure for isolating intact chloroplasts from plant leaves. The procedure involves mechanical cell wall and cell membrane breakage, removal of cell debris and leaf tissue by filtration, collection of total cell chloroplast fraction by centrifugation, and separation of intact chloroplasts from broken chloroplasts using a Percoll® layer or gradient.

Zastosowanie

Chloroplast Isolation Kit may be used in chloroplast organelle research to isolate intact chloroplasts from plant leaves for structural and functional studies.

Cechy i korzyści

  • Specifically formulated buffer - Saves time using easy to follow simple and proven protocols
  • Isolates intact chloroplasts - Useful for studies of such processes as carbon assimilation, electron flow and phosphorylation, metabolic transport or protein targeting
  • Total organelle protein complement - Useful for functional studies, metabolic transport, proteomic profiling, and genomic applications
  • Convenient format - Saves time and reduces waste

Przydatność

The Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco.
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Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki

  • Chloroplast Isolation Buffer 5x (CIB) 500 mL

  • A8022Bovine Serum Albumin, heat shock fraction, pH 5.2, ≥96% 3 gKarta charakterystyki

  • Chloroplast Isolation Buffer 5x (CIB) 4 ea

  • P4937Percoll®, pH 8.5-9.5 (25 °C), suitable for cell culture 100 mLKarta charakterystyki

Kod klasy składowania

10 - Combustible liquids


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