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GE17-0891-01

Sigma-Aldrich

Percoll®

Cytiva 17-0891-01, pack of 1 L

Synonim(y):

Percoll Density Gradient Media

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About This Item

Kod UNSPSC:
12352207
NACRES:
NA.75

Formularz

colloidal (Colloidal solution of silica coated with polyvinylpyrrolidone (PVP))
liquid (Suspension)
solution

opakowanie

pack of 1 L

producent / nazwa handlowa

Cytiva 17-0891-01

kolor

Colorless to slightly yellow

zakres pH

8.5-9.5

gęstość

1.13 g/cm3

Opis ogólny

Percoll® is a well-referenced media for density gradient centrifugation of cells, viruses, and subcellular particles. Percoll® is composed of colloidal silica coated with polyvinylpyrrolidone (PVP).

Percoll® is a low viscosity density gradient medium for preparation of cells, subcellular particles, and larger viruses. The low viscosity of the medium enables cell preparation on preformed gradients in only a few minutes using low centrifugal forces (200 to 1000 × g). The medium is available in easy-to-open, resealable 250 mL and 1 l bottles.

Percoll® consists of silica particles (15 to 30 nm diameter) coated with non-dialyzable polyvinylpyrrolidone (PVP). Free PVP is present at only 1% to 2%. Percoll® is non-toxic, almost chemically inert and does not adhere to membranes. Percoll® gradients can be formed within the density range of 1.0 to 1.3 g/mL, and are iso-osmotic throughout.

Percoll® can be stored unopened at room temperature for five years. At -20° C, it can only be stored for up to six months. If stored at -20° C, gradients form upon thawing, necessitating a mixing of the bottle before use. Preformed gradients can be stored for weeks without a change in gradient shape, provided that the gradient remains unfrozen. Percoll® can be buffered within the pH range 5.5 to 10.0 without any changes in properties. If the pH is dropped below 5.5, gelling may occur. Gelling can also be caused by the presence of divalent cations, an effect which is exacerbated by elevated temperatures.

Zastosowanie

Used in balanced salt solutions, physiological saline or 0.25 M sucrose.

Cechy i korzyści

  • For separation of cells, subcellular particles and larger viruses (down to ~ 70S) under gentle conditions which preserve viability and morphological integrity.
  • Non-toxic to cells.
  • Adjustable to physiological ionic strength and pH.
  • Gradients can either be preformed or spontaneously generated by centrifugation at moderate speeds in an angle-head rotor.
  • Gradients are iso-osmotic throughout and cover a range of densities up to 1.3 g/mL.

Przechowywanie i stabilność

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Komentarz do analizy

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informacje prawne

Percoll is a registered trademark of Cytiva
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number

Przepraszamy, ale COA dla tego produktu nie jest aktualnie dostępny online.

Proszę o kontakt, jeśli potrzebna jest pomoc Obsługa Klienta

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Qun Wang et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(45), 28212-28220 (2020-10-28)
Somatic mutations are major genetic contributors to cancers and many other age-related diseases. Many disease-causing somatic mutations can initiate clonal growth prior to the appearance of any disease symptoms, yet experimental models that can be used to examine clonal abnormalities
Joseph R Albe et al.
PLoS pathogens, 15(6), e1007833-e1007833 (2019-06-21)
Rift Valley fever virus (RVFV) causes severe disease in livestock concurrent with zoonotic transmission to humans. A subset of people infected with RVFV develop encephalitis, and significant gaps remain in our knowledge of how RVFV causes pathology in the brain.
Ailan Zhang et al.
Cellular and molecular gastroenterology and hepatology, 10(2), 309-326 (2020-04-25)
In short-bowel syndrome (SBS), inadequate intestinal adaptation is responsible for the majority of complications, including sepsis, liver failure, and death. In this study, we sought to further delineate the adaptive response to identify potential therapeutic targets. We performed a 75%
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Protokoły

Jak tworzyć i stosować gradienty Percoll

Aby przygotować gradient Percolla, osmolalność Percolla musi być dostosowana za pomocą soli fizjologicznej lub pożywki do hodowli komórkowej, aby Percoll był izotoniczny z fizjologicznymi roztworami soli.

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