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Merck

CELLMM2

Millipore

FLAG® M Purification Kit

For Mammalian expression systems.

Synonim(y):

Anti-ddddk, Anti-dykddddk

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
12352200
NACRES:
NA.32

Poziom jakości

metody

protein extraction: suitable

Warunki transportu

wet ice

temp. przechowywania

−20°C

Zastosowanie

The FLAG Purification Kit utilizes CelLytic Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG® M2 affinity gel for affinity purification of active FLAG fusion proteins.

Learn more product details in our FLAG® application portal.

Cechy i korzyści

  • Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
  • ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
  • Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).

Opakowanie

Sufficient for 3-5 uses of 1-ml affinity purification column.

Inne uwagi

Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells.

Informacje prawne

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
This page may contain text that has been machine translated.

Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki

  • C2978CelLytic M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.Karta charakterystyki

  • SAE0194Purified 3xFLAG® peptide, ≥95% (HPLC), lyophilized powderKarta charakterystyki

  • A2220ANTI-FLAG® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solutionKarta charakterystyki

  • C2103Chromatography columns, general-purpose, volume 10 mL, Overall H 5 in.Karta charakterystyki

Kod klasy składowania

10 - Combustible liquids


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

M D Mostaqul Huq et al.
Molecular & cellular proteomics : MCP, 6(4), 677-688 (2007-01-09)
Retinoic acid receptors (RARs) belong to the nuclear receptor superfamily. The mechanism of ligand-dependent activation of RARs is well known. The effect of protein phosphorylation on the activity of RARs has also been demonstrated. However, it is unclear whether other
Takeshi Into et al.
Molecular and cellular biology, 28(4), 1338-1347 (2007-12-19)
Nitric oxide (NO) has been thought to regulate the immune system through S nitrosylation of the transcriptional factor NF-kappaB. However, regulatory effects of NO on innate immune responses are unclear. Here, we report that NO has a capability to control
Severa Bunda et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(36), E3785-E3794 (2014-08-27)
Mutations in Ras GTPase and various other components of the Ras signaling pathways are among the most common genetic alterations in human cancers and also have been identified in several familial developmental syndromes. Over the past few decades it has
Amitava Mukherjee et al.
PLoS pathogens, 7(3), e1001311-e1001311 (2011-03-26)
The host innate immune response to viral infections often involves the activation of parallel pattern recognition receptor (PRR) pathways that converge on the induction of type I interferons (IFNs). Several viruses have evolved sophisticated mechanisms to attenuate antiviral host signaling
Weiming Xu et al.
Molecules (Basel, Switzerland), 17(10), 12086-12101 (2012-10-23)
Recent genetic studies have shown that PCSK9, one of the key genes in cholesterol metabolism, plays a critical role by controlling the level of low-density lipoprotein receptor. However, how PCSK9 mediates LDLR degradation is still unknown. By combining a shotgun

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