DMS 53

95062823, human lung, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: DMS 53, 95062823
• pochodzenie biologiczne: human lung
• tryb wzrostu: Adherent
• kariotyp: Modal no. 60
• morfologia: Epithelial
• produkty: Variety of hormones, high level of calcitonin
• receptory: Bombesin (BN), Epidermal growth factor (EGF), Transforming growth factor (TGF-beta), acetylcholine receptor (AChR)
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian lung small cell carcinoma

Opis linii komórkowej

DMS 53 is a small cell carcinoma of the lung (SCLC) isolated in 1974 from a mediastinal biopsy at time of diagnosis from a 54 year-old male patient prior to treatment. DMS 53 secrete a large variety of hormones including adrenocorticotropin (ACTH), bombesin (BN), calcitonin (CT), chorionic gonadotrophin (hCG), glucagon, growth hormone (GH), oxytocin-neurophysin (OT-NP), parathyroid hormone (PTH), somatostatin-like immunoreactivity (SRIF), thyroid releasing hormone (TRH) and 17β-estradiol. Expression of cell surface antigens Leu-7, Class I HLA, CLASS II HLA and My23 (defined by AML-2-23 monoclonal antibody) has been shown. DMS 53 cells produce receptors for BN, epidermal growth factor (EGF), transforming growth factor-β (TGF β) and acetylcholine (AChR). This cell line was shown to be tumourigenic in nude, athymic mice.

Zastosowanie

Human lung tumour model

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 10
D16S539: 12,13
D5S818: 10,11
D7S820: 8,11
THO1: 8,9.3
TPOX: 12
vWA: 15,17

pożywka hodowlana

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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