RT4

91091914

• Synonim(y): RT-4 Cells, RT4PCells
• Kod UNSPSC: 41106514

Właściwości

• pochodzenie biologiczne: human bladder
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Epithelial
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian bladder transitional-cell carcinoma

Opis linii komórkowej

Derived from explants of a recurring papillary tumour of the bladder.

Zastosowanie

RT4 cell line has been used to study the effects of uropathogenic Escherichia coli on neutrophil adhesion via neutrophil adhesion assay.

Tumourigenicity studies

Profil DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8
D16S539: 9
D5S818: 11,12
D7S820: 9,12
THO1: 9,9.3
TPOX: 8,11
vWA: 14,17

pożywka hodowlana

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Inne uwagi

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